Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus

Autor:	Ralf J. Sommer, Xiaoyue Wang, Benjamin Schlager, Georg Braach
Rok vydání:	2009
Předmět:	Nematoda Transgene Recombinant Fusion Proteins Mutant ved/biology.organism_classification_rank.species Green Fluorescent Proteins Molecular Sequence Data Locus (genetics) Biology Molecular cloning chemistry.chemical_compound Endocrinology Transformation Genetic Genetics Animals Amino Acid Sequence Transgenes Cloning Molecular Genes Dominant ved/biology fungi Cell Biology Helminth Proteins DNA Helminth biology.organism_classification Transformation (genetics) Luminescent Proteins Pristionchus pacificus Nematode chemistry Mutation Collagen DNA Plasmids
Zdroj:	Genesis (New York, N.Y. : 2000). 47(5)
ISSN:	1526-968X
Popis:	We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus. genesis 47:300–304, 2009. © 2009 Wiley-Liss, Inc.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79109392984299adcd79e56172c15b47 https://pubmed.ncbi.nlm.nih.gov/19298013 Zobrazit plný text záznamu Plný text