Age, environment, object recognition and morphological diversity of GFAP-immunolabeled astrocytes
| Autor: | Pedro Fernando da Costa Vasconcelos, Cesar Augusto Raiol Foro, Cristovam Wanderley Picanço Diniz, Marcia Consentino Kronka Sosthenes, João Bento-Torres, Marcus Augusto de Oliveira, Daniel Guerreiro Diniz, Camila Mendes de Lima, Daniel C. Anthony |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aging Cognitive Neuroscience Hipocampo / fisiologia Population Hippocampus Environment Meio Ambiente Mice 03 medical and health sciences Behavioral Neuroscience Cognition 0302 clinical medicine Prote?na Glial Fibrilar ?cida / metabolismo Astr?citos / patologia Memory Glial Fibrillary Acidic Protein Astr?citos / metabolismo Animals Efeito Idade Giro Denteado / metabolismo Cognitive decline education Exercise Biological Psychiatry Giro Denteado / citologia Environmental enrichment education.field_of_study Glial fibrillary acidic protein biology Perforant Pathway Research Dentate gyrus Age Factors Hipocampo / citologia Astrocytes morphology General Medicine Phenotype 030104 developmental biology Astrocytes Cogni??o / fisiologia Dentate Gyrus biology.protein Mem?ria / fisiologia Female Psychology Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC Behavioral and Brain Functions : BBF |
| Popis: | Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. / University of Oxford. Department of Pharmacology. Laboratory of Experimental Neuropathology. Oxford, England, UK. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University of Oxford. Department of Pharmacology. Laboratory of Experimental Neuropathology. Oxford, England, UK. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brasil. / University of Oxford. Department of Pharmacology. Laboratory of Experimental Neuropathology. Oxford, England, UK. Background: Few studies have explored the glial response to a standard environment and how the response may be associated with age-related cognitive decline in learning and memory. Here we investigated aging and environ mental influences on hippocampal-dependent tasks and on the morphology of an unbiased selected population of astrocytes from the molecular layer of dentate gyrus, which is the main target of perforant pathway. Results: Six and twenty-month-old female, albino Swiss mice were housed, from weaning, in a standard or enriched environment, including running wheels for exercise and tested for object recognition and contextual memories. Young adult and aged subjects, independent of environment, were able to distinguish familiar from novel objects. All experimental groups, except aged mice from standard environment, distinguish stationary from displaced objects. Young adult but not aged mice, independent of environment, were able to distinguish older from recent objects. Only young mice from an enriched environment were able to distinguish novel from familiar contexts. Unbiased selected astrocytes from the molecular layer of the dentate gyrus were reconstructed in three-dimensions and classified using hierarchical cluster analysis of bimodal or multimodal morphological features. We found two morphologi cal phenotypes of astrocytes and we designated type I the astrocytes that exhibited significantly higher values of morphological complexity as compared with type II. Complexity = [Sum of the terminal orders + Number of termi nals] ? [Total branch length/Number of primary branches]. On average, type I morphological complexity seems to be much more sensitive to age and environmental influences than that of type II. Indeed, aging and environmental impoverishment interact and reduce the morphological complexity of type I astrocytes at a point that they could not be distinguished anymore from type II. Conclusions: We suggest these two types of astrocytes may have different physiological roles and that the det rimental effects of aging on memory in mice from a standard environment may be associated with a reduction of astrocytes morphological diversity. |
| Databáze: | OpenAIRE |
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