N-Glycan structures of an osteopontin from human bone
| Autor: | Noriko Takahashi, Yoshinori Tsukamoto, Katsuyoshi Masuda, Hideki Honma, Kenjiro Kohri |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Glycan
Sialoglycoproteins Molecular Sequence Data Biophysics Human bone Aminopyridines Bone matrix Mass spectrometry Biochemistry High-performance liquid chromatography Bone and Bones Mass Spectrometry Polysaccharides Humans Tissue Distribution Osteopontin Molecular Biology Chromatography High Pressure Liquid biology Chemistry Cell Biology carbohydrates (lipids) Carbohydrate Sequence Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein |
| Zdroj: | Biochemical and biophysical research communications. 268(3) |
| ISSN: | 0006-291X |
| Popis: | N -Glycan structures of osteopontin (a bone matrix protein) from human bone (lumbar vertabrate) are reported in detail. Asn-linked glycan portion was released from 100 μg of osteopontin by digestion with glycoamidase A (from sweet almond), and the reducing ends of the N -glycans were reductively aminated with 2-aminopyridine. The derivatized N -glycans were separated and structurally identified by a multidimensional mapping technique on HPLC columns. Two major N -glycan structures were also confirmed by mass spectrometry. The proposed structures are shown below. The result should permit future comparison with the N -glycan structures of osteopontins obtained from other sources (kidney tissues, macrophages, urinary stones, human milk, etc.). |
| Databáze: | OpenAIRE |
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