Effect of zinc on carp (Cyprinus carpio L.) erythrocytes
| Autor: | Roman Gondko, Teresa Gabryelak, Zofia Jóźwiak, Anna Akahori |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Carps
Membrane Fluidity Immunology Acid Phosphatase Lipid Bilayers chemistry.chemical_element Zinc In Vitro Techniques Cell Fractionation Hemolysis Nucleic Acids Membrane fluidity Animals Sulfhydryl Compounds Carp Lipid bilayer Fluorescent Dyes Pharmacology Differential centrifugation chemistry.chemical_classification biology Erythrocyte Membrane biology.organism_classification Catalase In vitro Succinate Dehydrogenase Membrane chemistry Biochemistry Thiol Electrophoresis Polyacrylamide Gel Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Comparative biochemistry and physiology. Part C, Pharmacology, toxicologyendocrinology. 123(3) |
| ISSN: | 1367-8280 |
| Popis: | The effect of zinc exposure on some properties of the carp erythrocyte membrane was studied in vitro. Red blood cells plasma membranes were separated from other cellular membranes using a combination of differential and density gradient centrifugation. The purity of obtained plasma membrane preparations was determined by measuring the activity of the marker enzymes. Electrophoretic patterns of the main erythrocyte membrane proteins excluded their degradation during the isolation and purification procedure. Carp erythrocyte membranes, obtained from cells previously incubated with increasing ZnSO 4 concentrations, were used to elucidate the effect of zinc ions on their physical and biochemical properties. Using fluorescent probes: 12-AS and TMA–DPH, we found that zinc ions reduced the fluidity of the lipid bilayer, both in the middle and near the aqueous interface. Moreover, it was observed that zinc had no significant influence neither on the Na,K-ATPase activity nor on the thiol groups content in the erythrocyte membrane. We also detected that incubation of erythrocytes with zinc lead to the marked decrease of hemolytic resistance of the cells. Our studies demonstrate that zinc at higher concentrations may be toxic to carp erythrocytes causing changes in the membrane fluidity and hemolytic resistance. |
| Databáze: | OpenAIRE |
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