Transforming growth factor beta 1 (TGF beta 1) gene expression by eosinophils in asthmatic airway inflammation
| Autor: | Isao Ohno, Masaaki Honma, Kunio Shirato, Yuko Nitta, Kohei Yamauchi, Hiroki Hoshi, Paul M. O'Byrne, Gen Tamura, Manel Jordana, K L Woolley |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Clinical Biochemistry Gene Expression Bronchi HL-60 Cells Inflammation In situ hybridization Pathogenesis Transforming Growth Factor beta Gene expression medicine Humans RNA Messenger Molecular Biology In Situ Hybridization TGF beta 1 Basement membrane biology Histocytochemistry Cell Biology Transforming growth factor beta Middle Aged Asthma Eosinophils medicine.anatomical_structure Immunology biology.protein Female medicine.symptom Myofibroblast |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 15:404-409 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.15.3.8810646 |
| Popis: | The increase in thickness of bronchial walls by such structural changes as subepithelial fibrosis contributes to the severity and chronicity of asthma by amplifying airway narrowing. However, the pathogenesis of this structural alteration is not known. Transforming growth factor beta 1 (TGF beta 1) is known to have biologic activities relevant to the cellular and molecular events in subepithelial fibrosis, such as the deposition of collagen I and III and the increase of myofibroblasts beneath the epithelial basement membrane. Therefore, we examined TGF beta 1 gene expression in bronchial biopsy tissues from five severe asthmatics, five mild asthmatics, and five normal subjects using in situ hybridization combined with histochemical staining. Cells expressing TGF beta 1 mRNA were detected in tissues from four normal subjects, one mild asthmatic, and five severe asthmatics. The density of positive cells in severe asthmatic tissues (52.1 +/- 22.7, mean +/- SD/mm2) was significantly greater than that in mild asthmatic tissues (1.0 +/- 1.9/mm2, P0.01) or normal tissues (10.5 +/- 10.6/mm2, P0.02). The density in mild asthmatic tissues was not significantly different from that in normal tissues. The vast majority of positive cells in severe (99.1 +/- 1.7%) and mild (100%) asthmatic tissues were identified as eosinophils. In contrast, eosinophils constituted a small portion of positive cells (20.8 +/- 21.6%) in normal tissues. These results indicated that TGF beta 1 mRNA was overexpressed in severe asthmatics and that the main source of the mRNA was eosinophils, suggesting that eosinophils play an important role in the pathogenesis not only of inflammation but also of structural changes, such as subepithelial fibrosis, in asthmatic airways. |
| Databáze: | OpenAIRE |
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