Activation of tracheal smooth muscle responsiveness by fMLP-treated HL-60 cells and neutrophils
| Autor: | P. J. Cozzi, Julian Solway, Nilda M. Munoz, Alan R. Leff, A J Vita, S. L. Baranowski, K. J. Hamann |
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| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Contraction (grammar) Physiology Cell Survival Neutrophils Bronchoconstriction Guinea Pigs Indomethacin Lipoxygenase Stimulation Cell Communication Biology Physiology (medical) Internal medicine Muscarinic acetylcholine receptor Acetamides medicine Tumor Cells Cultured Animals Humans Lipoxygenase Inhibitors Respiratory system Phenyl Ethers Muscle Smooth Cell Biology Immunohistochemistry In vitro Acetylcholine N-Formylmethionine Leucyl-Phenylalanine Trachea Endocrinology Cell culture Leukemia Myeloid Prostaglandin-Endoperoxide Synthases Immunology medicine.symptom Muscle contraction medicine.drug |
| Zdroj: | The American journal of physiology. 264(3 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | We assessed the effects of cultured human promyelocytic leukemia (HL-60) cells and polymorphonuclear leukocytes (neutrophils) isolated from peripheral human blood on tracheal smooth muscle responsiveness in 40 male Hartley guinea pigs. Undifferentiated HL-60 cells (16-25 passages) were activated in vitro by incubation with 1 microM f-Met-Leu-Phe (fMLP), and force of contraction was measured isometrically using an in situ preparation of tracheal smooth muscle. Increasing concentrations of acetylcholine (ACh; 10(-10) to 10(-6) mol/cm2 tracheal surface) were applied topically to the epithelial surface pretreated with 4 x 10(6) fMLP-activated HL-60 cells, 4 x 10(6) fMLP-activated neutrophils, 4 x 10(6) sham-activated HL-60 cells, fMLP+vehicle, or vehicle control. Topical application of fMLP-activated HL-60 cells caused a maximum active tension (AT) of 1.13 +/- 0.2 g/cm after 5 min; fMLP-activated neutrophils, sham-activated HL-60 cells, or fMLP+vehicle had no effect. The fMLP-activated HL-60 cells also caused substantial augmentation of tracheal contraction to ACh (P < 0.05 vs. sham-activated cells for all concentrations > 10(-9) mol/cm2). Although fMLP treatment caused 247 +/- 28% increase from baseline level in O2-. production, neither direct contraction nor augmentation of muscarinic stimulation was demonstrated after topical application of 4 x 10(6) neutrophils. In 12 other preparations, fMLP-activated HL-60 cells were pretreated with either 10 microM indomethacin (Indo) or 100 microM A63162, a 5-lipoxygenase inhibitor. Pretreatment with Indo caused complete blockade of direct tracheal contraction and 88 +/- 13% blockade of muscarinic augmentation; there was no effect after A63162.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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