Establishment and characterization of an immortalized human giant congenital melanocytic nevi cell line

Autor:	Qingxiong, Yu, Rehanguli, Aimaier, Man-Hon, Chung, Xiwei, Cui, Yuehua, Li, Zhichao, Wang, Qingfeng, Li
Rok vydání:	2022
Předmět:	Nevus Pigmented Phosphatidylinositol 3-Kinases Skin Neoplasms Proto-Oncogene Proteins c-bcl-2 Oncology Humans Antineoplastic Agents Dermatology Protein Kinase Inhibitors Proto-Oncogene Proteins c-akt General Biochemistry Genetics and Molecular Biology Cell Line Phosphoinositide-3 Kinase Inhibitors
Zdroj:	Pigment Cell & Melanoma Research. 35:356-368
ISSN:	1755-148X 1755-1471
DOI:	10.1111/pcmr.13033
Popis:	Treatments for giant congenital melanocytic nevi (GCMN) are extremely limited. Thus, there is an urgent need for development of relevant targeted therapies. However, current lack of preclinical cell models restricts progress in GCMN research. In this study, we aimed to establish and characterize an immortalized GCMN cell line. GCMN cells were successfully immortalized by means of lentivirus-mediated simian virus 40 large T transfection. The immortalized GNC cell line (ImGNC) showed lower proliferation rate and higher melanin content than primary melanocytes. Expression levels of the differentiation gene MITF and stemness genes TWIST1, SNAI1, and FOXD3 were elevated in ImGNCs; however, the established ImGNC cell line was immortalized but not transformed. Sanger sequencing detected the heterozygous NRAS
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7907eebdba1c46596863fd0d13e885ad https://doi.org/10.1111/pcmr.13033 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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