Epidemiological investigations and molecular characterization of ‘candidatus phytoplasma solani’ in grapevines, weeds, vectors and putative vectors in western Sicily, (Southern Italy)
| Autor: | Gianfranco Romanazzi, Sergio Murolo, Gaetano Conigliaro, Elham Jamshidi, Vera D'Urso, S. Burruano, Patrizia Bella, Selene Giambra, Vincenzo Mondello, Gabriella Lo Verde, Haralabos Tsolakis |
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| Přispěvatelé: | Gaetano Conigliaro, Elham Jamshidi, Gabriella Lo Verde, Patrizia Bella, Vincenzo Mondello, Selene Giambra, Vera D’Urso, Haralabos Tsolaki, Sergio Murolo, Santella Burruano, Gianfranco Romanazzi |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Empoasca Phytoplasma Vineyard Article Botany tuf gene Immunology and Allergy Candidatus Phytoplasma solani vmp1 gene Grapevine yellows Molecular Biology General Immunology and Microbiology Molecular epidemiology biology Settore AGR/12 - Patologia Vegetale Vectors biology.organism_classification Vmp1 gene Infectious Diseases grapevine yellow Settore AGR/11 - Entomologia Generale E Applicata Tuf gene Grapevine Restriction fragment length polymorphism Weed vector |
| Zdroj: | Pathogens Volume 9 Issue 11 |
| Popis: | Bois noir is caused by &lsquo Candidatus Phytoplasma solani&rsquo and it is one of the most important and widespread diseases in the Euro-Mediterranean region. There are complex interactions between phytoplasma and grapevines, weeds, and vectors. These ecological relationships can be tracked according to molecular epidemiology. The aims of the 2-year study (2014&ndash 2015) were to describe incidence and spatial distribution of Bois noir in a vineyard with three grapevine varieties in Sicily, and to identify the molecular types of the tuf and vmp1 genes in these naturally infected grapevines, according to the potential reservoir plants and vectors. Disease incidence in 2015 was significantly higher in &lsquo Chardonnay&rsquo (up to 35%) than for &lsquo Nero d&rsquo Avola&rsquo and &lsquo Pinot noir&rsquo (< 5%). All grapevine, weed, and insect samples were infected by &lsquo Ca. P. solani&rsquo tuf-type b. Most of the collected insects were strictly related to Vitis spp. and belonged to Neoaliturus fenestratus, Empoasca spp., and Zygina rhamni. The characterization of the vmp1 gene revealed six different vmp types in grapevines (V1, V4, V9, V11, V12, V24), three in weeds (V4, V9, V11), and four in insects (V4, V9, V11, V24). Notably, V4, V9, appear both in hosts and vectors, with V9 predominant. Virtual restriction fragment length polymorphism (RFLP) analysis based on the nucleotide sequences supported the data of the conventional RFLP. Connections between the molecular data recorded in the vineyard ecosystems and the application of innovative tools based on the geostatistical analysis will contribute to further clarification of the specific ecological and epidemiological aspects of &lsquo in Sicily. |
| Databáze: | OpenAIRE |
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