Reconfigurable asymmetric protein assemblies through implicit negative design
| Autor: | Danny D. Sahtoe, Florian Praetorius, Alexis Courbet, Yang Hsia, Basile I. M. Wicky, Natasha I. Edman, Lauren M. Miller, Bart J. R. Timmermans, Justin Decarreau, Hana M. Morris, Alex Kang, Asim K. Bera, David Baker |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Models
Molecular Protein Folding Multidisciplinary Protein Conformation Proteins Crystallography X-Ray Protein Engineering Protein Subunits Multiprotein Complexes Escherichia coli Humans Computer Simulation Protein Conformation beta-Strand Protein Multimerization Protein Structure Quaternary HeLa Cells |
| Zdroj: | Science. 375 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Asymmetric multiprotein complexes that undergo subunit exchange play central roles in biology but present a challenge for design because the components must not only contain interfaces that enable reversible association but also be stable and well behaved in isolation. We use implicit negative design to generate β sheet–mediated heterodimers that can be assembled into a wide variety of complexes. The designs are stable, folded, and soluble in isolation and rapidly assemble upon mixing, and crystal structures are close to the computational models. We construct linearly arranged hetero-oligomers with up to six different components, branched hetero-oligomers, closed C4-symmetric two-component rings, and hetero-oligomers assembled on a cyclic homo-oligomeric central hub and demonstrate that such complexes can readily reconfigure through subunit exchange. Our approach provides a general route to designing asymmetric reconfigurable protein systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|