Immunochemical studies of factor V of bovine platelets
| Autor: | Razia Rawala, Robert W. Colman, T. Roy Ittyerah |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Blood Platelets
medicine.medical_treatment Immunoelectrophoresis In Vitro Techniques Biochemistry Plasma Thrombin medicine Animals Platelet Gel electrophoresis Antiserum Protease biology medicine.diagnostic_test Molecular mass Chemistry Immunochemistry Factor V Molecular biology biology.protein Cattle Collagen medicine.drug Peptide Hydrolases Subcellular Fractions |
| Zdroj: | European journal of biochemistry. 120(2) |
| ISSN: | 0014-2956 |
| Popis: | Immunochemical techniques were employed to investigate the molecular properties of bovine platelet factor V. An antiserum prepared against purified factor V rapidly inactivated platelet factor V, indicating that platelet factor V is antigenically related to plasma factor V. A reaction of identity between factor V in plasma and purified factor V was documented by immunodiffusion against an antibody to platelet factor V. When platelet factor V was extracted with Triton X-100 in the presence of protease inhibitors and subjected to immunoelectrophoresis against the antiserum to plasma factor V, a single antigenic component migrating toward the anode was observed. In the absence of protease inhibitors, following release by collagen or solubilization, platelet factor V appeared close to the origin suggesting proteolytic alteration. Platelet factor V released by collagen or extracted with Triton X-100 was activated by thrombin 7.4-fold and 4.5-fold respectively, compared to a 17-fold activation of factor V in plasma under identical conditions. The ability of thrombin to activate platelet factor V as well as the close correspondence of factor V activity and antigen released by collagen indicates that the molecule is largely in the unactivated form after release. A single component of molecular weight 270 000 was seen when platelet factor V released by collagen was immunoprecipitated and subjected to dodecylsulphate gel electrophoresis. Factor V coagulant assays and immunoelectrophoresis of subcellular fractions showed that platelet factor V is localized primarily in the alpha granules. Platelet factor V appears to be similar to, if not identical with, plasma factor V by the criteria of immunologic identity, similar electrophoretic mobility and virtually identical molecular weights. |
| Databáze: | OpenAIRE |
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