UFM1 suppresses invasive activities of gastric cancer cells by attenuating the expression of PDK1 through PI3K/AKT signaling

Autor:	Ying-Hong Yang, Jun Lu, Xin-Sheng Xie, Xiong-Feng Weng, Jian-Wei Xie, Long-Long Cao, Sheng-Liang Qiu, Jian-Xian Lin, Qi-Yue Chen, Mi Lin, Ru-Hong Tu, Ning-Zi Lian, Jia-Bin Wang, Chang-Ming Huang, Ping Li, Chang Hwan Yoon, Chao-Hui Zheng
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Cancer Research lcsh:RC254-282 Metastasis 03 medical and health sciences 0302 clinical medicine Nude mouse medicine Protein kinase B PI3K/AKT/mTOR pathway Gene knockdown biology business.industry UFM1 EMT Cancer medicine.disease biology.organism_classification lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Real-time polymerase chain reaction Oncology PDK1 030220 oncology & carcinogenesis Cancer cell Cancer research business Gastric cancer
Zdroj:	Journal of Experimental & Clinical Cancer Research, Vol 38, Iss 1, Pp 1-16 (2019)
ISSN:	1756-9966
Popis:	Background UFM1 has been found to be involved in the regulation of tumor development. This study aims to clarify the role and potential molecular mechanisms of UFM1 in the invasion and metastasis of gastric cancer. Methods Expression of UFM1 in gastric tumor and paired adjacent noncancerous tissues from 437 patients was analyzed by Western blotting, immunohistochemistry, and realtime PCR. Its correlation with the clinicopathological characteristics and prognosis of gastric cancer patients was analyzed. The effects of UFM1 on the invasion and migration of gastric cancer cells were determined by the wound and trans-well assays, and the effect of UFM1 on subcutaneous tumor formation was verified in nude mice. The potential downstream targets of UFM1 and related molecular mechanisms were clarified by the human protein kinase assay and co-immunoprecipitation technique. Results Compared with the corresponding adjacent tissues, the transcription level and protein expression level of UFM1 in gastric cancer tissues were significantly downregulated (P P Conclusion UFM1 suppresses the invasion and metastasis of gastric cancer by increasing the ubiquitination of PDK1 through negatively regulating PI3K/AKT signaling.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7903ab7e0df493c2da6c83a2037c0bd7 http://link.springer.com/article/10.1186/s13046-019-1416-4 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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