Fetal rat lung type II cell differentiation in serum-free isolated cell culture: modulation and inhibition
| Autor: | T. Lacaze-Masmonteil, Caroline Fraslon, V. Zupan, Jacques R. Bourbon, B. Chailley-Heu |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Pulmonary Surfactant-Associated Proteins Time Factors Hydrocortisone Physiology Mesenchyme Proteolipids Immunoblotting Enzyme-Linked Immunosorbent Assay Gestational Age Lamellar granule Biology Acetates Culture Media Serum-Free Dexamethasone Epithelium Choline Fetus Pulmonary surfactant Transforming Growth Factor beta Physiology (medical) medicine Animals Rats Wistar Lung Cells Cultured Phospholipids Basement membrane Cell Differentiation Epithelial Cells Pulmonary Surfactants Cell Biology Molecular biology Lipids Surfactant protein A Rats Chemically defined medium Kinetics Microscopy Electron Mifepristone medicine.anatomical_structure Cell culture Culture Media Conditioned Female |
| Zdroj: | The American journal of physiology. 264(5 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | Undifferentiated fetal rat lung epithelial cells were isolated on gestational days 15 or 17 (term 22 days) and cultured in a defined medium. On plastic, most of the cells developed structurally abnormal lamellar bodies. On a basement membrane matrix (BMM), they sequentially accumulated glycogen and formed typical lamellar bodies. Biochemical analysis of the latter indicated that they had a phospholipid composition typical of surfactant for cells on BMM but not on plastic and that surfactant protein A appeared on BMM only. Progressing maturation from day 1 to day 6 in culture was demonstrated for 17-day cells on BMM by a sevenfold increase of labeled precursor incorporation into surfactant phospholipids. Exposure to medium conditioned by 21-day fetal fibroblasts enhanced incorporation already after a 1-day culture. The antisteroid RU 486 had no effect on differentiation, whereas transforming growth factor-beta, a factor produced by lung mesenchyme at early fetal stages, inhibited it markedly. Alveolar epithelial type II cells appear to be committed early, but their maturational process would be prevented until a definite gestational stage. |
| Databáze: | OpenAIRE |
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