Culture of fetal cells from maternal blood for prenatal diagnosis
| Autor: | Sherman Elias, Hélène A. Gussin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Prenatal diagnosis Cell Separation In situ hybridization Prenatal care Biology law.invention Mesoderm Andrology Fetus Pregnancy law Prenatal Diagnosis medicine Humans Endothelium Metaphase Cells Cultured Polymerase chain reaction Chromosome Aberrations Erythroid Precursor Cells Blood Cells Stem Cells Obstetrics and Gynecology medicine.disease Reproductive Medicine Cell-free fetal DNA embryonic structures Chromosome abnormality Female |
| Zdroj: | Human Reproduction Update. 8:523-527 |
| ISSN: | 1460-2369 1355-4786 |
| DOI: | 10.1093/humupd/8.6.523 |
| Popis: | The isolation and analysis of fetal cells from maternal blood would allow non-invasive prenatal genetic screening and diagnosis. Over the past decade, progress has been made towards this goal using various enrichment strategies and analysis by fluorescence in-situ hybridization with chromosome-specific probes and PCR. One method that is currently being explored involves culturing fetal cells. Developing conditions which allow the number of fetal-derived cells to expand in culture and the number of maternally derived cells to be suppressed in culture may lead to a new selection process for obtaining fetal cells. Culturing of fetal cells from maternal blood could make possible conventional metaphase analysis of fetal cells for diagnosis of chromosomal abnormalities. |
| Databáze: | OpenAIRE |
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