Comparison of in vitro cell cytotoxic assays for tumor necrosis factor
| Autor: | George E. Gifford, David A. Flick |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Cytotoxicity
Immunologic Pathology medicine.medical_specialty Neutral red Time Factors Necrosis Immunology Cell Cell Count Biology Mice chemistry.chemical_compound L Cells medicine Animals Immunology and Allergy Cytotoxic T cell Cytotoxicity Glycoproteins Mice Inbred BALB C Dactinomycin Dose-Response Relationship Drug Tumor Necrosis Factor-alpha Cytotoxicity Tests Immunologic Molecular biology Staining medicine.anatomical_structure chemistry Tumor necrosis factor alpha Rabbits medicine.symptom medicine.drug |
| Zdroj: | Journal of Immunological Methods. 68:167-175 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(84)90147-9 |
| Popis: | Four published in vitro assays which measure cell cytotoxicity were compared utilizing murine tumor necrosis factor. These included determination of residual cell number by crystal violet staining in the presence and absence of actinomycin D, lack of viability as determined by neutral red uptake, and [3H]thymidine release in cytotoxin treated L929 cells. Treatment of cells with actinomycin D followed by crystal violet staining was the most sensitive method measured. However, addition of actinomycin D to the neutral red uptake assay could be shown to be even more sensitive. Additionally, it was shown how actinomycin D dosage, cell seeding density and time of incubation affect TNF titer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|