Lariciresinol induces apoptosis in HepG2 cells via mitochondrial-mediated apoptosis pathway
| Autor: | Gang Su, Guohu Chen, Li Lu, Han-mei Sun, Meng-yuan Wang, Xue-Xi Wang, Zhanjun Ma, Zhuan-ling Wang, Jingjing Yang, Yang Yang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Cell Survival Apoptosis Lignans Flow cytometry 03 medical and health sciences 0302 clinical medicine Western blot Annexin medicine Humans Cytotoxic T cell Furans Cells Cultured Caspase Cell Proliferation Membrane Potential Mitochondrial Pharmacology biology medicine.diagnostic_test Chemistry Cell growth Cytochrome c Hep G2 Cells Molecular biology Mitochondria 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | European Journal of Pharmacology. 821:1-10 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2017.12.027 |
| Popis: | Lariciresinol (LA) is one of the main active ingredients in many traditional medicinal plants such as Patrinia, and has the role of anti-liver cancer. However, the precise mechanisms are unclear. This study investigated the molecular mechanisms of LA against HepG2 cells. LA anti-tumor activity was assessed with the CCK-8, Ki-67, and immunofluorescence staining. Cells apoptotic ratio was evaluated by Annexin V/PI double-staining assay. A proteomic approach was used to identify differentially expressed proteins after LA treatment. JC-1 staining was carried out to detect the mitochondrial membrane potential (ΔΨm), and the Western blot analysis was used to analyse the apoptosis-associated proteins. Our results suggested that LA significantly suppressed the viability of HepG2 cells. The CCK-8 and Ki-67 expression indicated dose-dependent decreases in cell proliferation. Flow cytometry analysis showed that LA exhibited a apoptosis-inducing effect. The proteomic study observed the presence of apoptosis-associated proteins and mitochondrial dysfunction in HepG2 cells after LA-treatment. Further analysis showed that LA could trigger the mitochondrial-mediated apoptosis pathway, based on a decrease in ΔΨm; deliver of cytochrome c; activation of caspase-9/-3 and poly(ADP-ribose) polymerase; and decrease of the proportion of Bcl-2/Bax. Collectively, our studies found that LA exhibits significant cytotoxic effects by inhibiting cell proliferation, inducing apoptosis, possibly via activation of the mitochondrial-mediated apoptosis pathway. |
| Databáze: | OpenAIRE |
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