Differential incorporation of 3H-thymidine into DNA in cultured skin fibroblasts derived from patients with cystic fibrosis and controls
| Autor: | Louis F. H. Lam, Burton L. Shapiro |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Adolescent Cystic Fibrosis Biology Cystic fibrosis General Biochemistry Genetics and Molecular Biology Cycle time chemistry.chemical_compound Internal medicine medicine Humans General Pharmacology Toxicology and Pharmaceutics Child Mitosis Cultured skin General Medicine DNA Cell cycle Fibroblasts medicine.disease Molecular biology Endocrinology chemistry Female Thymidine Cell Division |
| Zdroj: | Life sciences. 24(26) |
| ISSN: | 0024-3205 |
| Popis: | Tritiated thymidine (3H-TdR) incorporation into DNA of the fibroblasts derived from subjects with cystic fibrosis (CF) and their controls was studied with scintillation counting and autoradiography. 3H-TdR incorporation at 24 hours postseeding was significantly less (p 0.1). The cell cycle time and the duration of each phase were studied by a mitotic selection and scintillation counting technique. There was no difference in cell cycle time between CF and control fibroblasts, however, the duration of the synthetic phase was significantly (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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