Signaling pathways associated with structural changes in varicose veins: a case–control study
| Autor: | Mohammad Mahdi Kamyar, Sakineh Amoueian, Mohamad Hadi Saeed Modaghegh, Shirin Saberianpour |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Pilot Projects 030204 cardiovascular system & hematology medicine.disease_cause Nitric oxide Varicose Veins Pathogenesis Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Varicose veins Humans Medicine Saphenous Vein Vein biology business.industry General Medicine Malondialdehyde 030104 developmental biology medicine.anatomical_structure chemistry Case-Control Studies biology.protein medicine.symptom Cardiology and Cardiovascular Medicine business Elastin Oxidative stress Signal Transduction |
| Zdroj: | Phlebology: The Journal of Venous Disease. 37:33-41 |
| ISSN: | 1758-1125 0268-3555 |
| DOI: | 10.1177/02683555211019537 |
| Popis: | Introduction and objectives In varicose veins, blood pressure increases in the veins of the lower extremities due to mechanical stimulation and function remodeling. The aim of this study is assessment of Signaling pathways associated with structural changes in varicose veins. Materials and methods This pilot study was performed on patients with varicose veins, which had undergone surgery. The healthy tissues from trauma patients or vascular bypass without underlying diseases were used for control samples. Hematoxylin-eosin, trichrome, and elastin staining were used for histopathological examination. The levels of MDA (malondialdehyde), total thiol, SOD (Superoxide dismutase) and NO (nitric oxide) level were measured using Elisa kits to evaluate the oxidative stress level. Gene expression levels of MMP2, MMP9, FOXO3a, APOE and p53 genes were determined using Real-time PCR. Results This study showed, the vascular Vein wall changes are visible in vascular collagen staining. Although these changes are observed in the structure of vascular wall collagen fibers, the accumulation of collagen and elastin was increased in the walls of varicose veins compared to the control group. The amount of nitric oxide and thiol were increased in the varicose group (P Conclusion This study demonstrated changes in oxidative stress, morphological structure, and aging pathways in varicose when compared to non-varicose veins. The changes in oxidative stress may be associated with the variations in morphological structure and aging pathways which contribute to the pathogenesis of varicose veins. |
| Databáze: | OpenAIRE |
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