Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan
| Autor: | Abu Baker Siddique, Mohsin Khurshid, Muhammad Hidayat Rasool, Rukhsana Yousaf, Muhammad Saqalein |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Phage therapy medicine.medical_treatment 030106 microbiology Clear Plaques MRSA Screen test medicine.disease_cause Microbiology Bacteriophage 03 medical and health sciences Antibiotic resistance In-Vitro Efficacy medicine Cefoxitin In-Vivo Model biology business.industry biology.organism_classification Methicillin-resistant Staphylococcus aureus 030104 developmental biology Infectious Diseases Lytic cycle Staphylococcus aureus business medicine.drug Research Article SDS-PAGE |
| Zdroj: | Jundishapur Journal of Microbiology |
| ISSN: | 2008-4161 2008-3645 |
| Popis: | Background In recent years, antibiotic resistance has been indicated as a paramount threat to public health. The use of bacteriophages appears to be a safer alternative for the control of bacterial infections. Objectives The present study aims to explore sewage water for the presence of indigenous bacteriophages, and to investigate their antibacterial potential against Methicillin-resistant Staphylococcus aureus (MRSA). Methods Bacterial isolates were first collected and identified from pus samples taken from the surgical and burn units using standard microbiological procedures. A cefoxitin disk screen test was then used and interpreted according to the clinical laboratory standards institute (CLSI) guidelines for the detection of MRSA. The sewage samples were processed and the phages enriched using S. aureus as a host organism. Turbid and clear plaques of different sizes were isolated using an overlay method, purified, and then enumerated by means of a dilution method. Results The phages exhibited good lytic activity against MRSA when tested in-vitro, and the highest activity was attained within three to six hours of phage infection. The isolated phage pq/48 was also found efficient in decreasing the bacterial count during an in-vivo trial in rabbits. A protein analysis using SDS-PAGE revealed 10 proteins of between 20 kDa and 155 kDa in size. Conclusions The overall results indicated that bacteriophages isolated from sewage exhibited excellent lytic activity against MRSA strains. In conclusion, bacteriophages can be further characterized and appear to be a promising candidate for phage therapy against MRSA in the future. |
| Databáze: | OpenAIRE |
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