Implantable controlled delivery systems for proteins based on collagen--pHEMA hydrogels
| Autor: | D. Vijaya Ramesh, K. Panduranga Rao, Jyoti K. Rao |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Materials science
Biophysics Serum albumin Bioengineering Biocompatible Materials macromolecular substances (Hydroxyethyl)methacrylate In Vitro Techniques complex mixtures Redox Biomaterials chemistry.chemical_compound Polymer chemistry Animals Bovine serum albumin Fluorescein isothiocyanate Polyhydroxyethyl Methacrylate Drug Implants biology technology industry and agriculture Serum Albumin Bovine Cross-Linking Reagents chemistry Mechanics of Materials Drug delivery Self-healing hydrogels Ceramics and Composites biology.protein Microscopy Electron Scanning Cattle Collagen Ethylene glycol Gels Oxidation-Reduction Fluorescein-5-isothiocyanate Nuclear chemistry |
| Zdroj: | Biomaterials. 15(5) |
| ISSN: | 0142-9612 |
| Popis: | Co-polymeric hydrogels of collagen and poly(hydroxyethyl methacrylate) (pHEMA) were prepared using an ammonium persulphate-sodium metabisulphite redox initiation technique. Fluorescein isothiocyanate (FITC)-labelled bovine serum albumin (BSA) was entrapped into these hydrogels. These gels were modified differently by external cross-linking and made highly porous by incorporation of ethylene glycol. The morphological characteristics of the gels containing FITC-BSA before and after release were studied. The in vitro release of FITC-labelled BSA was studied in phosphate buffer, pH 7.4, at 37 degrees C. The results clearly indicated that collagen-pHEMA hydrogels release FITC-BSA in a zero-order fashion. In the case of uncross-linked hydrogels, the release of FITC-BSA was about 81% within 8 d, compared to 49% for the same period in the case of cross-linked hydrogels. It was also observed that in the case of porous hydrogels the release was very fast (19% in the first hour) and lasted for about 12 h, releasing 76% of FITC-BSA. These results clearly indicated that collagen-pHEMA hydrogels released BSA in a zero-order fashion and that the release rate can be programmed by appropriate modification of the hydrogel matrices. |
| Databáze: | OpenAIRE |
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