Acute regulated expression of pendrin in human urinary exosomes

Autor: Lydie Cheval, Carsten A. Wagner, Ganesh Pathare, Nasser A. Dhayat, Thomas J. Neuhaus, Nilufar Mohebbi, Daniel Guido Fuster
Přispěvatelé: University of Zurich, Fuster, Daniel G, Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Fluides Réactifs et Turbulence (IJLRDA-FRT), Institut Jean le Rond d'Alembert (DALEMBERT), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
MESH: Salt Stress
MESH: Acidosis
Renal Tubular
Physiology
[SDV]Life Sciences [q-bio]
Clinical Biochemistry
030232 urology & nephrology
1308 Clinical Biochemistry
Exosomes
Salt Stress
Acid-base homeostasis
10052 Institute of Physiology
0302 clinical medicine
MESH: Bicarbonates
2737 Physiology (medical)
Distal renal tubular acidosis
Ingestion
Homeostasis
Urinary exosomes
10035 Clinic for Nephrology
MESH: Ammonia
Receptor
610 Medicine & health
biology
Chemistry
Venous blood
Acidosis
Renal Tubular
Sulfate Transporters
MESH: Homeostasis
Adult
medicine.medical_specialty
Urinary system
Acid–base homeostasis
Excretion
03 medical and health sciences
Ammonia
Physiology (medical)
Internal medicine
medicine
otorhinolaryngologic diseases
SLC26A4
Humans
Pendrin
MESH: Exosomes
MESH: Humans
MESH: Adult
1314 Physiology
medicine.disease
MESH: Sulfate Transporters
MESH: Male
Bicarbonates
030104 developmental biology
Endocrinology
biology.protein
MESH: Biomarkers
570 Life sciences
Biomarkers
Zdroj: Pflügers Archiv European Journal of Physiology
Pflügers Archiv European Journal of Physiology, Springer Verlag, 2018, 470 (2), pp.427-438. ⟨10.1007/s00424-017-2049-0⟩
Pathare, Ganesh Tukaram; Dhayat, Nasser; Mohebbi, Nilufar; Wagner, Carsten A.; Cheval, Lydie; Neuhaus, Thomas J.; Fuster, Daniel Guido (2018). Acute regulated expression of pendrin in human urinary exosomes. Pflügers Archiv : European journal of physiology, 470(2), pp. 427-438. Springer 10.1007/s00424-017-2049-0
ISSN: 0031-6768
1432-2013
DOI: 10.1007/s00424-017-2049-0⟩
Popis: International audience; It is well known that pendrin, an apical Cl-/HCO3-exchanger in type B intercalated cells, is modulated by chronic acid-base disturbances and electrolyte intake. To study this adaptation further at the acute level, we analyzed urinary exosomes from individuals subjected to oral acute acid, alkali, and NaCl loading. Acute oral NH4Cl loading (n = 8) elicited systemic acidemia with a drop in urinary pH and an increase in urinary NH4 excretion. Nadir urinary pH was achieved 5 h after NH4Cl loading. Exosomal pendrin abundance was dramatically decreased at 3 h after acid loading. In contrast, after acute equimolar oral NaHCO3 loading (n = 8), urinary and venous blood pH rose rapidly with a significant attenuation of urinary NH4 excretion. Alkali loading caused rapid upregulation of exosomal pendrin abundance at 1 h and normalized within 3 h of treatment. Equimolar NaCl loading (n = 6) did not alter urinary or venous blood pH or urinary NH4 excretion. However, pendrin abundance in urinary exosomes was significantly reduced at 2 h of NaCl ingestion with lowest levels observed at 4 h after treatment. In patients with inherited distal renal tubular acidosis (dRTA), pendrin abundance in urinary exosomes was greatly reduced and did not change upon oral NH4Cl loading. In summary, pendrin can be detected and quantified in human urinary exosomes by immunoblotting. Acid, alkali, and NaCl loadings cause acute changes in pendrin abundance in urinary exosomes within a few hours. Our data suggest that exosomal pendrin is a promising urinary biomarker for acute acid-base and volume status changes in humans.
Databáze: OpenAIRE
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