Multipotent stem/progenitor cells in the human foetal biliary tree
| Autor: | Cristina Napoli, Paolo Onori, Antonio Franchitto, Maurizio M. Anceschi, Alfredo Cantafora, Eugenio Gaudio, Vincenzo Cardinale, Rossella Semeraro, A. Torrice, Roberto Brunelli, Daniela Bosco, Raffaele Gentile, Domenico Alvaro, Guido Carpino, Lola M. Reid |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
pancreas endodermal stem cells biliary tree stem cells liver biliary tree human foetal liver Cellular differentiation Mice SCID In Vitro Techniques Biology Mice Fetus Bile Ducts Extrahepatic Internal medicine medicine Animals Humans Progenitor cell Biliary Tract Induced pluripotent stem cell Pancreas Cells Cultured Cell Proliferation Hepatology Multipotent Stem Cells Cell Differentiation Cell biology Endothelial stem cell Transplantation Bile Ducts Intrahepatic Phenotype medicine.anatomical_structure Endocrinology Multipotent Stem Cell embryonic structures Hepatocytes Stem cell |
| Popis: | Background & Aims Biliary tree, liver, and pancreas share a common embryological origin. We previously demonstrated the presence of stem/progenitor cells of endodermal origin in the adult human extrahepatic biliary tree. This study evaluated the human foetal biliary trees as sources of stem/progenitor cells of multiple endodermal-derived mature fates. Methods Human foetal intrahepatic and extrahepatic biliary tree tissues and isolated cells were tested for cytoplasmic and surface markers of stem cells and committed progenitors, as well as endodermal transcription factors requisite for a liver versus pancreatic fate. In vitro and in vivo experiments were conducted to evaluate the potential mature fates of differentiation. Results Foetal biliary tree cells proliferated clonogenically for more than 1month on plastic in a serum-free Kubota medium. After culture expansion, cells exhibited multipotency and could be restricted to certain lineages under defined microenvironments, including hepatocytes, cholangiocytes, and pancreatic islet cells. Transplantation of foetal biliary tree cells into the livers of immunodeficient mice resulted in effective engraftment and differentiation into mature hepatocytes and cholangiocytes. Conclusions Foetal biliary trees contain multipotent stem/progenitor cells comparable with those in adults. These cells can be easily expanded and induced in vitro to differentiate into liver and pancreatic mature fates, and engrafted and differentiated into mature cells when transplanted in vivo . These findings further characterise the development of these stem/progenitor cell populations from foetuses to adults, which are thought to contribute to liver and pancreas organogenesis throughout life. |
| Databáze: | OpenAIRE |
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