Interleukin 4 deficiency limits the development of a lupus‐like disease in mice triggered by phospholipids in a non‐bilayer arrangement
| Autor: | Rogelio Hernández-Pando, Irene Nevárez-Lechuga, Alejandro Escobar-Gutiérrez, Albany Reséndiz-Mora, Isabel Baeza, Carlos Wong-Baeza, Carla Landa-Saldívar, Eréndira Molina-Gómez, Isabel Wong-Baeza |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class medicine.medical_treatment Lipid Bilayers Immunology Cell Monoclonal antibody Mice 03 medical and health sciences Th2 Cells 0302 clinical medicine stomatognathic system medicine Animals Lupus Erythematosus Systemic Phospholipids Interleukin 4 Autoantibodies Mice Knockout Mice Inbred BALB C Kidney Liposome Systemic lupus erythematosus biology Chemistry Antibodies Monoclonal General Medicine medicine.disease Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Immunoglobulin G biology.protein Female Interleukin-4 Antibody 030215 immunology |
| Zdroj: | Scandinavian Journal of Immunology. 93 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/sji.13002 |
| Popis: | Non-bilayer phospholipids arrangements (NPAs) are transient molecular associations different from lipid bilayers. When they become stable, they can trigger a disease in mice resembling human lupus, which is mainly characterized by the production of anti-NPA IgG antibodies. NPAs are stabilized on liposomes or cell bilayers by the drugs procainamide or chlorpromazine, which produce drug-induced lupus in humans. Here, we evaluated the participation of the TH 2 response, through its hallmark cytokine IL-4, on the development of the lupus-like disease in mice. Wild-type or IL-4 knockout BALB/c mice received liposomes bearing drug-induced NPAs, the drugs alone, or an anti-NPA monoclonal antibody (H308) to induce the lupus-like disease (the last two procedures stabilize NPAs on mice cells). IL-4 KO mice showed minor disease manifestations, compared to wild-type mice, with decreased production of anti-NPA IgG antibodies, no anti-cardiolipin, anti-histones and anticoagulant antibodies, and no kidney or skin lesions. In these mice, H308 was the only inducer of anti-NPA IgG antibodies. These findings indicate that IL-4 has a central role in the development of the murine lupus-like disease induced by NPA stabilization. |
| Databáze: | OpenAIRE |
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