Action Mode of the Mycotoxin Patulin as a Novel Natural Photosystem II Inhibitor
| Autor: | Reto J. Strasser, Shiguo Chen, He Wang, Sheng Qiang, Hazem M. Kalaji, Liu Weizhe, Xiaoxiong Wang, Yanjing Guo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll
0106 biological sciences animal structures Photosystem II 010401 analytical chemistry Photosystem II Protein Complex food and beverages Plastoquinone General Chemistry Photosynthetic efficiency 01 natural sciences 0104 chemical sciences Electron Transport Patulin chemistry.chemical_compound chemistry Biophysics Phytotoxicity Photosynthesis General Agricultural and Biological Sciences Mode of action Mycotoxin Chlorophyll fluorescence 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:7313-7323 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.1c01811 |
| Popis: | A biocontrol method plays an important role in weed management. In this study, we aimed to clarify the phytotoxicity of the mycotoxin patulin (PAT) and reveal its mode of action as a new natural photosystem II (PSII) inhibitor. Phytotoxicity test showed that PAT has herbicidal activity and causes significant leaf lesions on Ageratina adenophora. Under a half-inhibition concentration I50 (2.24 μM), the observed significant decrease in oxygen evolution rate and the increase in the J-step of the chlorophyll fluorescence rise OJIP curve indicated that PAT strongly reduces photosynthetic efficiency by blocking electron transport from the primary to secondary plastoquinone acceptors (QA to QB) of PSII. Molecular modeling of PAT docking to the A. adenophora D1 protein suggested that PAT bounds to the QB site by forming hydrogen bonds to histidine 252 in the D1 protein. It is proposed that PAT is a new natural PSII inhibitor and has the potential to be developed into a bioherbicide or used as a template scaffold for discovering novel derivatives with more potent herbicidal activity. |
| Databáze: | OpenAIRE |
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