Micro-liquid chromatography mass spectrometry for the analysis of antineoplastic drugs from wipe samples
| Autor: | Jochen Tuerk, Terence Hetzel, Thorsten Teutenberg, Claudia vom Eyser, Torsten C. Schmidt |
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| Rok vydání: | 2016 |
| Předmět: |
Formic acid
Analytical chemistry Chemie Antineoplastic Agents Tandem mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liquid chromatography–mass spectrometry Limit of Detection Tandem Mass Spectrometry Desorption Occupational Exposure Workplace Detection limit Chromatography 010401 analytical chemistry Extraction (chemistry) Repeatability Microfluidic Analytical Techniques 030210 environmental & occupational health Ion source 0104 chemical sciences chemistry Calibration Environmental Pollutants Chromatography Liquid Environmental Monitoring |
| Zdroj: | Analytical and bioanalytical chemistry. 408(28) |
| ISSN: | 1618-2650 |
| Popis: | A fast quantification method for the determination of 11 antineoplastic drugs from wipe samples was developed using micro-scale liquid chromatography in combination with tandem mass spectrometry. The extraction efficiency from the wipes has been investigated using different extraction solvents. The results indicate that a mixture of 70/30 water/isopropanol (v/v) acidified with 0.1 % formic acid is suitable to desorb the antineoplastic drugs with sufficient recovery between 80 and 120 %. Compared to conventional liquid chromatography, the total analysis time can be reduced to 2.25 min using a 50 × 0.3 mm column at a flow rate of 25 μL min−1. Ion source parameters as well as the injection volume were optimized to ensure the highest sensitivity. The results of method validation showed an instrumental limit of quantification between 0.0068 and 0.0488 ng mL−1 using an injection volume of 4.25 μL estimated by the signal to noise ratio. Moreover, the retention time repeatability was determined with a maximum relative standard deviation of 0.4 %. |
| Databáze: | OpenAIRE |
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