Presence of a covalently linked protein on calicivirus RNA
| Autor: | F. Brown, J. N. Burroughs |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Infectivity
Swine Polyacrylamide Calicivirus RNA Biology Virology Molecular biology Virus Cell Line Molecular Weight Electrophoresis chemistry.chemical_compound Viral Proteins Biochemistry chemistry Covalent bond Mole Animals RNA Viral Vesicular Exanthema of Swine Peptides Caliciviridae Peptide Hydrolases |
| Zdroj: | The Journal of general virology. 41(2) |
| ISSN: | 0022-1317 |
| Popis: | Summary The infective RNA of the calicivirus, vesicular exanthema virus, has been shown to contain a protein which is apparently linked to the RNA by a covalent bond. The protein remained bound to the RNA after boiling with SDS-mercaptoethanol-urea or treating with formamide-dimethylsulphoxide but was removed by incubating with proteinase K. The mol. wt. of the protein was estimated to be about 10 × 103 by electrophoresis in highly cross-linked polyacrylamide gels. The infectivity of the RNA was destroyed by removal of the protein with proteinase K. |
| Databáze: | OpenAIRE |
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