Converting Mosquito Surveillance to Arbovirus Surveillance with Honey-Baited Nucleic Acid Preservation Cards
| Autor: | Stephen L. Doggett, Cheryl S. Toi, Emily J. Flies, Philip Weinstein, Craig R. Williams |
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| Přispěvatelé: | Flies, Emily J, Toi, Cheryl, Weinstein, Philip, Doggett, Stephen L, Williams, Craig R |
| Rok vydání: | 2015 |
| Předmět: |
Mosquito Control
Public health interventions Alphavirus Biology Arbovirus Infections Microbiology Arbovirus Ross River virus Spatio-Temporal Analysis Virology parasitic diseases medicine High spatial resolution Animals Humans Stratford virus Saliva mosquitoes saliva Transmission (medicine) fungi transmission Australia food and beverages Honey biology.organism_classification medicine.disease Insect Vectors arbovirus Infectious Diseases Culicidae Vector (epidemiology) surveillance RNA Viral Barmah Forest virus Sentinel Surveillance Arboviruses |
| Zdroj: | Vector borne and zoonotic diseases (Larchmont, N.Y.). 15(7) |
| ISSN: | 1557-7759 |
| Popis: | Spatially and temporally accurate information about infectious mosquito distribution allows for pre-emptivepublic health interventions that can reduce the burden of mosquito-borne infections on human populations.However, the labile nature of arboviruses, the low prevalence of infection in mosquitoes, the expensive laborcosts for mosquito identification and sorting, and the specialized equipment required for arbovirus testing canobstruct arbovirus surveillance efforts. The recently developed techniques of testing mosquito expectorate usinghoney-baited nucleic acid preservation cards or sugar bait stations allows a sensitive method of testing forinfectious, rather than infected, mosquito vectors. Here we report the results from the first large-scale incorporationof honey-baited cards into an existing mosquito surveillance program. During 4 months of the peakvirus season ( January–April, 2014) for a total of 577 trap nights, we set CO2-baited encephalitis vector survey(EVS) light traps at 88 locations in South Australia. The collection container for the EVS trap was modified toallow for the placement of a honey-baited nucleic acid preservation card (FTATM card) inside. After collection,mosquitoes were maintained in a humid environment and allowed access to the cards for 1 week. Cards werethen analyzed for common endemic Australian arboviruses using a nested RT-PCR. Eighteen virus detections,including 11 Ross River virus, four Barmah Forest virus, and three Stratford virus (not previously reported fromSouth Australia) were obtained. Our findings suggest that adding FTA cards to an existing mosquito surveillanceprogram is a rapid and efficient way of detecting infectious mosquitoes with high spatial resolution. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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