Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy
| Autor: | Regina Orzekowsky-Schroeder, Dorthe von Smolinski, Andreas Gebert, Maike Blessenohl, Gereon Huettmann, Anna Schueth, Antje Klinger, Norbert Koop |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cell type
Histology Enteroendocrine cell Biology Mice Imaging Three-Dimensional Microscopy Electron Transmission Two-photon excitation microscopy Intestinal mucosa Intestine Small medicine Animals Anesthesia Intestinal Mucosa Molecular Biology Mice Inbred BALB C Original Paper Lamina propria Microscopy Confocal Microvilli Cell migration Small intestine Cell Biology Cell biology Mucosal architecture Medical Laboratory Technology Autofluorescence Enterocytes medicine.anatomical_structure Microscopy Fluorescence Cellular migration Intravital two-photon microscopy Female |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-011-0905-0 |
| Popis: | The mucosa of the gastrointestinal tract is a dynamic tissue composed of numerous cell types with complex cellular functions. Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional imaging of the vital murine intestine in anaesthetized mice. Using intravital autofluorescence 2-photon (A2P) microscopy we studied the choreographed interactions of enterocytes, goblet cells, enteroendocrine cells and brush cells with other cellular constituents of the small intestinal mucosa over several hours at a subcellular resolution and in three dimensions. Vigorously moving lymphoid cells and their interaction with constituent parts of the lamina propria were examined and quantitatively analyzed. Nuclear and lectin staining permitted simultaneous characterization of autofluorescence and admitted dyes and yielded additional spectral information that is crucial to the interpretation of the complex intestinal mucosa. This novel intravital approach provides detailed insights into the physiology of the small intestine and especially opens a new window for investigating cellular dynamics under nearly physiological conditions. Electronic supplementary material The online version of this article (doi:10.1007/s00418-011-0905-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|