The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis
| Autor: | Kevin Kavanagh, James C. Carolan, Christine T. Griffin, Louise Mc Namara, David A. Fitzpatrick |
|---|---|
| Přispěvatelé: | Department of Agriculture, Food and the Marine, Science Foundation Ireland, 10/RD/MCOP/NUIM/720, 12/RI/2346 (3) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Metarhizium Microinjections Proteome Lawrge pine weevil Antimicrobial peptides Biological pest control Gene Expression Bassiana 01 natural sciences Biochemistry Microbiology Immunomodulation 03 medical and health sciences Hemolymph Candida albicans Animals Protease Inhibitors Hylobius abietis Beauveria Pest Control Biological Molecular Biology biology Weevil fungi Proteomic Biocontrol Pinus biology.organism_classification 010602 entomology Entomopathogenic fungi 030104 developmental biology Culture Media Conditioned Larva Insect Science comic_books Metarhizium brunneum Insect Proteins Weevils Transcriptome Filtration comic_books.character Antimicrobial Cationic Peptides |
| Popis: | peer-reviewed The large pine weevil Hylobius abietis L. is a major forestry pest in 15 European countries, where it is a threat to 3.4 million hectares of forest. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of H. abietis was performed. Injection with Metarhizium brunneum or Beauvaria bassiana culture filtrate facilitated a significantly increased yeast cell proliferation in larvae. Larvae co-injected with either Beauvaria caledonica or B. bassiana culture filtrate and Candida albicans showed significantly increased mortality. Together these results suggest that EPF culture filtrate has the potential to modulate the insect immune system allowing a subsequent pathogen to proliferate. Injection with EPF culture filtrate was shown to alter the abundance of protease inhibitors, detoxifing enzymes, antimicrobial peptides and proteins involved in reception/detection and development in H. abietis larvae. Larvae injected with B. caledonica culture filtrate displayed significant alterations in abundance of proteins involved in cellulolytic and other metabolic processes in their haemolymph proteome. Screening EPF for their ability to modulate the insect immune response represents a means of assessing EPF for use as biocontrol agents, particularly if the goal is to use them in combination with other control agents. This research was funded by the Irish Government (Department of Agriculture, Food and the Marine) (10/RD/MCOP/NUIM/720) under the National Development Plan 2007–2013 and through the MU Department of Biology Contingency Fund. The Q-Exactive quantitative mass spectrometer was funded under the SFI Research Infrastructure Call 2012; Grant Number: 12/RI/2346 (3) to Prof. S. Doyle. |
| Databáze: | OpenAIRE |
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