The role of disulfide bond isomerase A (DsbA) ofEscherichia coliO157:H7 in biofilm formation and virulence
| Autor: | Yunho Lee, Che Ok Jeon, Younghoon Kim, Sujin Yeom, Sungsu Park, Woojun Park, Sae Hun Kim |
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| Rok vydání: | 2008 |
| Předmět: |
animal diseases
Green Fluorescent Proteins Protein Disulfide-Isomerases Virulence Escherichia coli O157 medicine.disease_cause Microbiology Bacterial Adhesion chemistry.chemical_compound fluids and secretions Menadione Oxidoreductase Cell Line Tumor Genetics medicine Animals Humans Caenorhabditis elegans Molecular Biology Escherichia coli chemistry.chemical_classification Models Genetic biology Escherichia coli Proteins Biofilm Vitamin K 3 Gene Expression Regulation Bacterial Periplasmic space Hydrogen-Ion Concentration biochemical phenomena metabolism and nutrition Alkaline Phosphatase Blotting Northern bacterial infections and mycoses Luminescent Proteins DsbA chemistry Biofilms Mutation biology.protein bacteria Alkaline phosphatase |
| Zdroj: | FEMS Microbiology Letters. 278:213-222 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2007.00993.x |
| Popis: | The role of periplasmic disulfide oxidoreductase DsbA in Shiga toxin-producing Escherichia coli O157:H7 (STEC) was investigated. Deletion of dsbA (DeltadsbA) significantly decreased cell motility and alkaline phosphatase activity in STEC. STEC DeltadsbA also showed greater sensitivity to menadione and under low pH conditions. Significant reductions in surface attachment to both biotic (HT-29 epithelial cells) and abiotic (polystyrene and polyvinyl chloride) surfaces were observed in STEC DeltadsbA. In addition, no biofilm formation was detected in STEC DeltadsbA compared to wild-type cells in glass capillary tubes under continuous flow-culture system conditions. In the nematode model Caenorhabditis elegans-killing assay, the deletion of dsbA in STEC resulted in attenuated virulence compared to wild-type cells. STEC DeltadsbA was also found to have a reduced ability to colonize the nematode gut. These results suggest that DsbA plays important roles in biofilm formation and virulence in STEC cells. |
| Databáze: | OpenAIRE |
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