Implant Materials Generate Different Peri-implant Inflammatory Factors

Autor: Jennifer M. Schneider, Paul J. Slosar, Sharon L. Hyzy, Rene Olivares-Navarrete, Zvi Schwartz, Barbara D. Boyan
Rok vydání: 2015
Předmět:
Polymers
Epidemiology
Apoptosis
Biocompatible Materials
02 engineering and technology
Polyethylene Glycols
Extracellular matrix
0302 clinical medicine
Fibrosis
Peek
Orthopedics and Sports Medicine
Cells
Cultured
Titanium
implant surface
Ti6Al4V
Biomaterial
Osteoblast
interbody spine cage
Ketones
021001 nanoscience & nanotechnology
medicine.anatomical_structure
Cellular Microenvironment
0210 nano-technology
medicine.medical_specialty
Surface Properties
inflammatory mediators
Bone and Bones
osteogenesis
Benzophenones
03 medical and health sciences
PEEK
Alloys
medicine
Humans
mRNA array
mesenchymal stem cells
business.industry
Interleukins
fibrosis
technology
industry
and agriculture
medicine.disease
Surgery
Cortical bone
Neurology (clinical)
Implant
Wound healing
business
030217 neurology & neurosurgery
DNA Damage
Biomedical engineering
Zdroj: Spine
ISSN: 0362-2436
DOI: 10.1097/brs.0000000000000778
Popis: An in vitro study examining factors produced by human mesenchymal stem cells on spine implant materials. Mesenchymal stem cells undergo differentiation to osteoblasts and secrete anti-inflammatory factors on micro-/nano-textured Ti alloy surfaces. Mesenchymal stem cells fail to differentiate on PEEK and produce high levels of proinflammatory factors.
Study Design. An in vitro study examining factors produced by human mesenchymal stem cells on spine implant materials. Objective. The aim of this study was to examine whether the inflammatory microenvironment generated by cells on titanium-aluminum-vanadium (Ti-alloy, TiAlV) surfaces is affected by surface microtexture and whether it differs from that generated on poly-ether-ether-ketone (PEEK). Summary of Background Data. Histologically, implants fabricated from PEEK have a fibrous connective tissue surface interface whereas Ti-alloy implants demonstrate close approximation with surrounding bone. Ti-alloy surfaces with complex micron/submicron scale roughness promote osteoblastic differentiation and foster a specific cellular environment that favors bone formation whereas PEEK favors fibrous tissue formation. Methods. Human mesenchymal stem cells were cultured on tissue culture polystyrene, PEEK, smooth TiAlV, or macro-/micro-/nano-textured rough TiAlV (mmnTiAlV) disks. Osteoblastic differentiation and secreted inflammatory interleukins were assessed after 7 days. Fold changes in mRNAs for inflammation, necrosis, DNA damage, or apoptosis with respect to tissue culture polystyrene were measured by low-density polymerase chain reaction array. Data were analyzed by analysis of variance, followed by Bonferroni's correction of Student's t-test. Results. Cells on PEEK upregulated mRNAs for chemokine ligand-2, interleukin (IL) 1β, IL6, IL8, and tumor necrosis factor. Cells grown on the mmnTiAlV had an 8-fold reduction in mRNAs for toll-like receptor-4. Cells grown on mmnTiAlV had reduced levels of proinflammatory interleukins. Cells on PEEK had higher mRNAs for factors strongly associated with cell death/apoptosis, whereas cells on mmnTiAlV exhibited reduced cytokine factor levels. All results were significant (P < 0.05). Conclusion. These results suggest that fibrous tissue around PEEK implants may be due to several factors: reduced osteoblastic differentiation of progenitor cells and production of an inflammatory environment that favors cell death via apoptosis and necrosis. Ti alloy surfaces with complex macro/micro/nanoscale roughness promote osteoblastic differentiation and foster a specific cellular environment that favors bone formation. Level of Evidence: N/A
Databáze: OpenAIRE
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