Differentially accessible, single copy sequences form contiguous domains along metaphase chromosomes that are conserved among multiple tissues
| Autor: | Seana L. Hill, Peter K. Rogan, Yi Xuan Wang, Joan H.M. Knoll |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Differential chromatin accessibility Cytogenomics Biology QH426-470 Biochemistry 03 medical and health sciences 0302 clinical medicine Metaphase homologous chromosomes Chromosome condensation Homologous chromosome medicine Genetics Molecular Biology Metaphase Mitosis Genetics (clinical) 030304 developmental biology 0303 health sciences Topologically associated domains (TADS) Research Biochemistry (medical) Cytogenetics Lymphocyte differentiation Chromosome Genomics Chromatin Cell biology Premature chromosome condensation Tissue/cell types Molecular Medicine Epigenetics 030217 neurology & neurosurgery |
| Zdroj: | Molecular Cytogenetics, Vol 14, Iss 1, Pp 1-17 (2021) Molecular Cytogenetics Biochemistry Publications |
| ISSN: | 1755-8166 |
| Popis: | BackgroundDuring mitosis, chromatin engages in a dynamic cycle of condensation and decondensation. Condensation into distinct units to ensure high fidelity segregation is followed by rapid and reproducible decondensation to produce functional daughter cells. Factors contributing to the reproducibility of chromatin structure between cell generations are not well understood. We investigated local metaphase chromosome condensation along mitotic chromosomes within genomic intervals showing differential accessibility (DA) between homologs. DA was originally identified using short sequence-defined single copy (sc) DNA probes of ResultsWe evaluated multiple adjacent scFISH probes at 6 different DA loci from chromosomal regions 2p23, 3p24, 12p12, 15q22, 15q24 and 20q13 within peripheral blood T-lymphocytes. DA was organized within domains that extend beyond the defined boundaries of individual scFISH probes. Based on hybridizations of 2 to 4 scFISH probes per domain, domains ranged in length from 16.0 kb to 129.6 kb. Transcriptionally inert chromosomal DA regions in T-lymphocytes also demonstrated conservation of DA in bone marrow and fibroblast cells.ConclusionsWe identified novel chromosomal regions with allelic differences in metaphase chromosome accessibility and demonstrated that these accessibility differences appear to be aggregated into contiguous domains extending beyond individual scFISH probes. These domains are encompassed by previously established topologically associated domain (TAD) boundaries. DA appears to be a conserved feature of human metaphase chromosomes across different stages of lymphocyte differentiation and germ cell origin, consistent with its proposed role in maintenance of intergenerational cellular chromosome memory. |
| Databáze: | OpenAIRE |
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