Tauopathy in the young autistic brain: novel biomarker and therapeutic target
| Autor: | Iris Grigg, Vlasta Korenková, Zlatko Marušić, Olga Touloumi, Roza Lagoudaki, Nikolaos Grigoriadis, Anke Van Dijck, Mirna Aničić, R. Frank Kooy, Jurica Vuković, Tom Aharon Hait, Illana Gozes, Yanina Ivashko-Pachima |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Autism Spectrum Disorder Nerve Tissue Proteins medicine.disease_cause Article lcsh:RC321-571 Cellular and Molecular Neuroscience Atrophy medicine Humans Autistic Disorder Child lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Biological Psychiatry Aged Homeodomain Proteins Mutation business.industry tauopathy ADNP autism DNA Helicases Brain Nuclear Proteins Diagnostic markers medicine.disease Nap Psychiatry and Mental health Tauopathies Autism spectrum disorder Biomarker (medicine) Autism Female Human medicine PAX6 Tauopathy business Neuroscience Biomarkers Transcription Factors |
| Zdroj: | Translational Psychiatry Translational psychiatry Translational Psychiatry, Vol 10, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2158-3188 |
| Popis: | Given our recent discovery of somatic mutations in autism spectrum disorder (ASD)/intellectual disability (ID) genes in postmortem aged Alzheimer’s disease brains correlating with increasing tauopathy, it is important to decipher if tauopathy is underlying brain imaging results of atrophy in ASD/ID children. We concentrated on activity-dependent neuroprotective protein (ADNP), a prevalent autism gene. The unique availability of multiple postmortem brain sections of a 7-year-old male, heterozygous for ADNP de novo mutation c.2244Adup/p.His559Glnfs*3 allowed exploration of tauopathy, reflecting on a general unexplored mechanism. The tested subject exhibited autism, fine motor delays, severe intellectual disability and seizures. The patient died after multiple organ failure following liver transplantation. To compare to other ADNP syndrome mutations, immortalized lymphoblastoid cell lines from three different patients (including ADNP p.Arg216*, p.Lys408Valfs*31, and p.Tyr719* heterozygous dominant mutations) and a control were subjected to RNA-seq. Immunohistochemistry, high-throughput gene expression profiles in numerous postmortem tissues followed. Comparisons to a control brain and to extensive datasets were used. Live cell imaging investigated Tau-microtubule interaction, protecting against tauopathy. Extensive child brain tauopathy paralleled by multiple gene expression changes was discovered. Tauopathy was explained by direct mutation effects on Tau-microtubule interaction and correction by the ADNP active snippet NAP. Significant pathway changes (empirical P value ADNP showed relatively reduced expression in the ADNP syndrome cerebellum, which was also observed for 25 additional genes (representing >50% of the tested genes), including NLGN1, NLGN2, PAX6, SMARCA4, and SNAP25, converging on nervous system development and tauopathy. NAP provided protection against mutated ADNP disrupted Tau-microtubule association. In conclusion, tauopathy may explain brain-imaging findings in ADNP syndrome children and may provide a new direction for the development of tauopathy protecting drug candidates like NAP in ASD/ID. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|