Effects of Spica prunellae on caspase-3-associated proliferation and apoptosis in human lung cancer cells in vitro
| Autor: | Meihui Liu, Yong Wang, Yuanfen Liu, Wei Zhang, Jinhua Zhu, Yiting Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Caspase 3 Apoptosis lcsh:RC254-282 Flow cytometry 03 medical and health sciences 0302 clinical medicine lung cancer cells A549 medicine Radiology Nuclear Medicine and imaging Viability assay Lung cancer medicine.diagnostic_test Chemistry Human lung cancer Spica prunellae General Medicine medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens In vitro Blot 030104 developmental biology Oncology Caspase-3 030220 oncology & carcinogenesis Cancer research |
| Zdroj: | Journal of Cancer Research and Therapeutics, Vol 14, Iss 4, Pp 760-763 (2018) |
| ISSN: | 1998-4138 0973-1482 |
| Popis: | Purpose: This study aimed to explore the molecular mechanisms underlying the effects of Spica prunellae on proliferation and apoptosis of lung cancer cells in vitro. Materials and Methods: The cell viability was determined with the CCK8 assay and the apoptosis was examined by flow cytometry. Western blotting was applied to detect the protein level of caspase-3 expression. Results: It was observed that Spica prunellae could promote the apoptosis and inhibit the proliferation of A549 in vitro. The analysis of Western blotting showed that the expression level of proapoptosis protein caspase-3 was generally unchanged, whereas the level of activated caspase-3 was significantly increased. Conclusion: The results indicated that the growth of lung cancer cells A549 might be inhibited with Spica prunellae through activating the proapoptotic protein caspase-3 and inducing cellular apoptotic pathway. |
| Databáze: | OpenAIRE |
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