The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco
| Autor: | Martina Zapletalová, Annick Chiltz, Zbyněk Zdráhal, Marek Petřivalský, Kamil Mikulášek, Nathalie Leborgne-Castel, Martin Solanský, Jan Lochman |
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| Přispěvatelé: | Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic, Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic, Department of Biochemistry, 3Department of Botany, Faculty of Science, Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech Republic, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Hypersensitive response hypersensitive response Physiology Nicotiana tabacum [SDV]Life Sciences [q-bio] oligomeric structure Plant Science 01 natural sciences Cell wall resistance 03 medical and health sciences Transduction (genetics) Tobacco elicitin response Amino Acid Sequence signalling 030304 developmental biology Plant Diseases 0303 health sciences biology Chemistry Pattern recognition receptor Elicitin phytophthora biology.organism_classification Cell biology suppressor of bir1-1 (SOBIR1) Oomycetes cell wall Phytophthora Solanaceae elicitin b-CRY 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2021, 72 (8), pp.3219-3234. ⟨10.1093/jxb/erab011⟩ |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Successful plant defence against microbial pathogens is based on early recognition and fast activation of inducible responses. Key mechanisms include detection of microbe-associated molecular patterns by membrane-localized pattern recognition receptors that induce a basal resistance response. A well-described model of such responses to pathogens involves the interactions between Solanaceae plants and proteinaceous elicitors secreted by oomycetes, called elicitins. It has been hypothesized that the formation of oligomeric structures by elicitins could be involved in their recognition and activation of defensive transduction cascades. In this study, we tested this hypothesis using several approaches, and we observed differences in tobacco plant responses induced by the elicitin β-cryptogein (β-CRY) and its homodimer, β-CRYDIM. We also found that the C-terminal domain of elicitins of other ELI (true-elicitin) clades plays a significant role in stabilization of their oligomeric structure and restraint in the cell wall. In addition, covalently cross-linking β-CRYDIM impaired the formation of signalling complexes, thereby reducing its capacity to elicit the hypersensitive response and resistance in the host plant, with no significant changes in pathogenesis-related protein expression. By revealing the details of the effects of β-CRY dimerization on recognition and defence responses in tobacco, our results shed light on the poorly understood role of elicitins’ oligomeric structures in the interactions between oomycetes and plants. |
| Databáze: | OpenAIRE |
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