Dimethylacetamide and trehalose for ram semen cryopreservation
| Autor: | Adrielle da Silva Lima, Antonio de Lisboa Ribeiro Filho, Rodrigo Freitas Bittencourt, Eunice Oba, Kárita da Mata Fuchs, Gabriel Felipe Oliveira de Menezes, Marta Vasconcelos Bittencourt, Hymerson Costa Azevedo, Carmo Emanuel Almeida Biscarde |
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| Přispěvatelé: | Universidade Federal da Bahia (UFBA), Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Rok vydání: | 2018 |
| Předmět: |
Amide
Male animal structures Cryoprotectant Semen General Biochemistry Genetics and Molecular Biology Cryopreservation Dimethylacetamide law.invention Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cryoprotective Agents law Acetamides Animals 030219 obstetrics & reproductive medicine Sheep urogenital system Extender 0402 animal and dairy science Trehalose 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sperm Semen cryopreservation Ovine Hyperosmotic extender Fertility chemistry Sperm Motility Frozen semen General Agricultural and Biological Sciences Semen Preservation |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1090-2392 |
| Popis: | Made available in DSpace on 2019-10-05T06:21:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%), and its interaction with trehalose (TRE, 100 mOsmol). A further objective of this study was to evaluate in vivo fertility of ram semen cryopreserved with dimethylacetamide. The control treatment was an extender medium with 6% glycerol (GLY). Five treatments were examined: 6% GLY, 3% DMA, 6% DMA, 3% DMA + TRE, and 6% DMA + TRE. After thawing, the kinetic sperm parameters were analyzed using a computerized system. Sperm viability was observed using a multiple parameter sperm staining with propidium iodide (for plasmatic membrane integrity), JC-1 (for mitochondrial membrane potential), and FITC-PSA (for acrosomal integrity). The isosmotic extender with GLY was the most effective medium for the maintenance of sperm characteristics, compared to extenders containing DMA as cryoprotectant. Regarding fertility, the extender medium with 3% DMA may be a safe alternative for sperm cryopreservation, and does not compromise the fertility rates. The addition of TRE decreased the kinetic sperm parameters; however, a positive effect on the integrity of the plasmatic and acrosomal membranes was observed. Univ Fed Bahia, Sch Vet & Anim Sci Med, Ave Adhemar de Barros 500, BR-40170110 Salvador, BA, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618000 Botucatu, SP, Brazil Embrapa Coastal Tablelands, Anim Reprod Biotechnol Lab, Ave Beira Mar 3250, BR-49025040 Aracaju, Sergipe, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-18618000 Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
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