SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice
| Autor: | Areum Lee, Hye-Sun Cho, Choonkyun Jung, Hyo-Jun Lee, Hae-Myeong Jung, Hyun Ji Park, Seung Hee Jo, Hyun-Soon Kim, Sung-Ran Min |
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| Rok vydání: | 2021 |
| Předmět: |
Spliceosome
QH301-705.5 RNA Splicing Mutant SUMO protein OsFKBP20-1b Catalysis Article Inorganic Chemistry heat stress Splicing factor Transcription (biology) Escherichia coli Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Plant Proteins Chemistry Organic Chemistry Alternative splicing fungi Sumoylation Oryza General Medicine Computer Science Applications Cell biology Cytoplasm splicing factor RNA splicing pre-mRNA splicing Heat-Shock Response |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 9049, p 9049 (2021) Volume 22 Issue 16 |
| ISSN: | 1422-0067 |
| Popis: | OsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the U snRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice. |
| Databáze: | OpenAIRE |
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