Detection of bacterial-reactive natural IgM antibodies in desert bighorn sheep populations

Autor: Melanie J Peel, Brian S. Dugovich, Aleksandra E. Sikora, Brian P. Dolan, Ryszard A. Zielke, Clinton W. Epps, Anna E. Jolles, Brianna R. Beechler, Amy L. Palmer
Rok vydání: 2017
Předmět:
0301 basic medicine
Desert bighorn sheep
Physiology
lcsh:Medicine
Wildlife
Biochemistry
Epitope
0302 clinical medicine
Immune Physiology
Microbial Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Mammals
Deserts
Immune System Proteins
Multidisciplinary
Ecology
Microbial Growth and Development
Ruminants
Terrestrial Environments
Body Fluids
Blood
Vertebrates
Anatomy
Desert Climate
Antibody
Research Article
Animal Types
Immunology
Sheep Diseases
Enzyme-Linked Immunosorbent Assay
Biology
Research and Analysis Methods
Microbiology
Ecosystems
Antibodies
Blood Plasma
03 medical and health sciences
Antigen
Animals
Immunoassays
Sheep
Plasma Proteins
Innate immune system
Vibrio coralliilyticus
Bacterial Growth
lcsh:R
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
030104 developmental biology
Immunoglobulin M
Amniotes
Immunologic Techniques
biology.protein
lcsh:Q
Bacterial antigen
Zoology
Developmental Biology
030215 immunology
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 6, p e0180415 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0180415
Popis: Ecoimmunology is a burgeoning field of ecology which studies immune responses in wildlife by utilizing general immune assays such as the detection of natural antibody. Unlike adaptive antibodies, natural antibodies are important in innate immune responses and often recognized conserved epitopes present in pathogens. Here, we describe a procedure for measuring natural antibodies reactive to bacterial antigens that may be applicable to a variety of organisms. IgM from desert bighorn sheep plasma samples was tested for reactivity to outer membrane proteins from Vibrio coralliilyticus, a marine bacterium to which sheep would have not been exposed. Immunoblotting demonstrated bighorn sheep IgM could bind to a variety of bacterial cell envelope proteins while ELISA analysis allowed for rapid determination of natural antibody levels in hundreds of individual animals. Natural antibody levels were correlated with the ability of plasma to kill laboratory strains of E. coli bacteria. Finally, we demonstrate that natural antibody levels varied in two distinct populations of desert bighorn sheep. These data demonstrate a novel and specific measure of natural antibody function and show that this varies in ecologically relevant ways.
Databáze: OpenAIRE
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