Rapid turnover of mannitol-1-phosphate in Escherichia coli
| Autor: | C. M. Hardy, S. M. Pearce, H. Rosenberg, P. A. Jacomb |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Monosaccharide Transport Proteins
chemistry.chemical_element Dehydrogenase Biology medicine.disease_cause Microbiology Phosphates chemistry.chemical_compound medicine Escherichia coli Fructosediphosphates Moiety Mannitol Phosphoenolpyruvate Sugar Phosphotransferase System Molecular Biology Phosphorus Escherichia coli Proteins digestive oral and skin physiology Mannitol Phosphates PEP group translocation Phosphate Kinetics chemistry Biochemistry Mutation Sugar Phosphates medicine.drug Research Article Sugar Alcohol Dehydrogenases |
| Popis: | The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate represents some 30% of this label. Mannitol-1-phosphate occurs in E. coli grown on a variety of carbon sources, in the absence of D-mannitol, and is synthesized de novo even in mutants lacking mannitol-1-phosphate dehydrogenase. The mannitol moiety of mannitol-1-phosphate was not affected during the total chase of the P moiety, which exchanged with a half-life of about 30 s. These findings suggest that the rapid equilibration of the phosphorus is a function of an enzyme, possibly a component of the phosphotransferase system, capable of forming a complex that allows the exchange of the phosphate without the equilibration of the mannitol moiety with free mannitol. |
| Databáze: | OpenAIRE |
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