Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
| Autor: | T, Conway, G W, Sewell, Y A, Osman, L O, Ingram |
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| Rok vydání: | 1987 |
| Předmět: |
Transcription
Genetic Dehydrogenase Molecular cloning Microbiology Zymomonas mobilis Gene product chemistry.chemical_compound Gram-Negative Bacteria Cloning Molecular Molecular Biology Alcohol dehydrogenase Regulation of gene expression Ethanol Base Sequence biology Alcohol Dehydrogenase Acetaldehyde Chromosome Mapping biology.organism_classification Molecular biology Gene Expression Regulation Genes Biochemistry chemistry Genes Bacterial biology.protein Research Article |
| Zdroj: | Journal of Bacteriology. 169:2591-2597 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.169.6.2591-2597.1987 |
| Popis: | The gene which encodes alcohol dehydrogenase II (adhB) from Zymomonas mobilis was cloned in Escherichia coli as a 1.4-kilobase DNA fragment by using a novel indicator plate which directly detects the expression of this activity by recombinant colonies. The DNA sequence for this clone contained an open reading frame encoding a polypeptide of 383 amino acids, with a molecular weight of 40,141. Although this protein exhibited very little homology with other known alcohol dehydrogenases, the predicted amino acid composition was in excellent agreement with that reported for the purified alcohol dehydrogenase II protein from Z. mobilis. In Z. mobilis, the adhB gene was transcribed from tandem promoters which were separated by 100 base pairs and ended with a transcriptional terminator (13-base-pair palindrome). In Escherichia coli, only one of the Z. mobilis promoters was used, despite apparent similarity to the enteric consensus promoter. The adhB gene was transcribed at low levels in E. coli from the P2 promoter of Z. mobilis but was expressed well in E. coli under control of the lac promoter (approximately 0.25% of the total cell protein). |
| Databáze: | OpenAIRE |
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