Is it the time to study air pollution effects under environmental conditions? A case study to support the shift of in vitro toxicology from the bench to the field
| Autor: | Luca Di Liberto, Claudia Consales, Giuseppe Raschellà, Gabriele Zanini, Michaela Aufderheide, Milena Stracquadanio, Maurizio Manigrasso, Maria Giuseppa Grollino, Francesca Costabile, Pasquale Avino, Ettore Petralia, Eugenia Cordelli, Francesca Pacchierotti, Maurizio Gualtieri, Alfred Wiedensohler |
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| Přispěvatelé: | Zanini, G., Pacchierotti, F., Stracquadanio, M., Raschellà, G., Petralia, E., Cordelli, E., Consales, C., Grollino, M. G., Gualtieri, M., Gualtieri, M, Grollino, M, Consales, C, Costabile, F, Manigrasso, M, Avino, P, Aufderheide, M, Cordelli, E, Di Liberto, L, Petralia, E, Raschella, G, Stracquadanio, M, Wiedensohler, A, Pacchierotti, F, Zanini, G |
| Rok vydání: | 2018 |
| Předmět: |
Pollution
Environmental Engineering 010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis media_common.quotation_subject Air pollution 010501 environmental sciences medicine.disease_cause 01 natural sciences Air liquid interface Correlation PM-Composition and biological effects In vitro environmental exposure Secondary aerosol Ultrafine particles toxicology Chemistry (all) Environmental Chemistry Air Pollution Ultrafine particle medicine Humans 0105 earth and related environmental sciences media_common Chemistry Public Health Environmental and Occupational Health In vitro toxicology Environmental Exposure General Medicine General Chemistry Environmental exposure Particulates Aerosol Correlation PM-Composition and biological effect Environmental chemistry |
| Zdroj: | Chemosphere. 207:552-564 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2018.05.130 |
| Popis: | Air pollution and particulate matter are recognised cause of increased disease incidence in exposed population. The toxicological processes underlying air pollution associated effects have been investigated by in vivo and/or in vitro experimentation. The latter is usually performed by exposing cells cultured under submerged condition to particulate matter concentration quite far from environmental exposure expected in humans. Here we report for the first time the feasibility of a direct exposure of air liquid interface cultured cells to environmental concentration of particulate matter. Inflammatory proteins release was analysed in cell medium while differential expression of selected genes was analysed in cells. Significant association of anti-oxidant genes was observed with secondary and aged aerosol, while cytochrome activation with primary and PAHs enriched ultrafine particles. The results obtained clearly show the opportunity to move from the lab bench to the field for properly understanding the toxicological effects also of ultrafine particles on selected in vitro models. © 2018 Elsevier Ltd |
| Databáze: | OpenAIRE |
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