Characterization of a Subset of the Basic-Helix-Loop-Helix-PAS Superfamily That Interacts with Components of the Dioxin Signaling Pathway
| Autor: | Victoria Jackiw, Yi Zhong Gu, R. Clark Brown, Marilyn G. Pray-Grant, John B. Hogenesch, Gary H. Perdew, William K. Chan, Christopher A. Bradfield |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Hypoxia-Inducible Factor 1
DNA Complementary Polychlorinated Dibenzodioxins Aryl hydrocarbon receptor nuclear translocator Immunoprecipitation Molecular Sequence Data Sequence alignment Biology Biochemistry Open Reading Frames PAS domain Basic Helix-Loop-Helix Transcription Factors Drosophila Proteins Humans Amino Acid Sequence RNA Messenger Cloning Molecular Enhancer Molecular Biology Basic helix-loop-helix Aryl Hydrocarbon Receptor Nuclear Translocator Helix-Loop-Helix Motifs Nuclear Proteins Cell Biology Hypoxia-Inducible Factor 1 alpha Subunit Ligand (biochemistry) Molecular biology Cell biology DNA-Binding Proteins Receptors Aryl Hydrocarbon Sequence Alignment Software Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 272:8581-8593 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.272.13.8581 |
| Popis: | In an effort to better understand the mechanism of toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin, we employed an iterative search of human expressed sequence tags to identify novel basic-helix-loop-helix-PAS (bHLH-PAS) proteins that interact with either the Ah receptor (AHR) or the Ah receptor nuclear translocator (ARNT). We characterized five new “embers f the AS superfamily,” or MOPs 1-5, that are similar in size and structural organization to the AHR and ARNT. MOPs 1-4 have N-terminal bHLH and PAS domains and C-terminal variable regions. MOP5 contained the characteristic PAS domain and a variable C terminus; it is possible that the cDNA contains a bHLH domain, but the entire open reading frame has yet to be completed. Coimmunoprecipitation studies, yeast two-hybrid analysis, and transient transfection experiments demonstrated that MOP1 and MOP2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo. MOP3 was found to associate with the AHR in vitro but not in vivo. This observation, coupled with the fact that MOP3 formed tighter associations with the 90-kDa heat shock protein than the human AHR, suggests that MOP3 may be a conditionally active bHLH-PAS protein that requires activation by an unknown ligand. The expression profiles of the AHR, MOP1, and MOP2 mRNAs, coupled with the observation that they all share ARNT as a common dimeric partner, suggests that the cellular pathways mediated by MOP1 and MOP2 may influence or respond to the dioxin signaling pathway. |
| Databáze: | OpenAIRE |
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