Expression of insulin-like growth factor binding proteins in human breast cancer correlates with estrogen receptor status
Autor: | William L. McGuire, James G. Jackson, Robert F. Krywicki, Douglas Yee, Jose A. Figueroa |
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Rok vydání: | 1993 |
Předmět: |
medicine.medical_specialty
medicine.drug_class Blotting Western Immunoblotting Estrogen receptor Breast Neoplasms Biology Biochemistry Insulin-like growth factor-binding protein Breast cancer Somatomedins Internal medicine Gene expression Tumor Cells Cultured medicine Humans RNA Messenger Receptor Molecular Biology Estrogen Receptor Status Cancer Cell Biology medicine.disease Insulin-Like Growth Factor Binding Proteins Endocrinology Receptors Estrogen Estrogen biology.protein Carrier Proteins hormones hormone substitutes and hormone antagonists |
Zdroj: | Journal of Cellular Biochemistry. 52:196-205 |
ISSN: | 1097-4644 0730-2312 |
Popis: | The insulin-like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the IGFs are associated with specific binding proteins (IGFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF-dependent growth. This study examined the expression of IGFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP-4 mRNA was detected in all BCCLs studied. IGFBP-5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP-6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47-D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2-6 but none expressed IGFBP-1 message. IGFBP-3 expression was higher in ER negative tumors, while that of IGFBP-4 and -5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology. |
Databáze: | OpenAIRE |
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