Salmonella vaccines secreting measles virus epitopes induce protective immune responses against measles virus encephalitis
| Autor: | Ivaylo Gentschev, Gerald Weidinger, Volker ter Meulen, Werner Goebel, Simone Spreng, Stefan Niewiesk |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Paramyxoviridae
Immunology Genetic Vectors Molecular Sequence Data Administration Oral Epitopes T-Lymphocyte Microbiology Epitope Measles virus Mice Morbillivirus Salmonella Animals Amino Acid Sequence Mononegavirales Mice Inbred C3H Attenuated vaccine biology Base Sequence Aroa Immunogenicity Viral Vaccines biology.organism_classification Virology Recombinant Proteins Infectious Diseases Epitopes B-Lymphocyte Subacute Sclerosing Panencephalitis Plasmids |
| Zdroj: | Microbes and infection. 2(14) |
| ISSN: | 1286-4579 |
| Popis: | In the present study we describe a live vaccine against measles virus (MV) infection on the basis of attenuated Salmonella typhimurium aroA secreting MV antigens via the Escherichia coli alpha-hemolysin secretion system. Two well-characterized MV epitopes, a B-cell epitope of the MV fusion protein (amino acids 404-414) and a T-cell epitope of the MV nucleocapsid protein (amino acids 79-99) were fused as single or repeating units to the C-terminal secretion signal of the E. coli hemolysin and expressed in secreted form by the attenuated S. typhimurium aroA SL7207. Immunization of MV-susceptible C3H mice revealed that S. typhimurium SL7207 secreting these antigens provoked a humoral and a cellular MV-specific immune response, respectively. Mice vaccinated orally with a combination of both recombinant S. typhimurium strains showed partial protection against a lethal MV encephalitis after intracerebral challenge with a rodent-adapted, neurotropic MV strain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect