An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites
| Autor: | Mohammed A. Al-Rawhani, David R. S. Cumming, Srinivas Velugotla, Michael P. Barrett, Bence Nagy, Boon Chong Cheah, Samadhan B. Patil, Dharmendra S. Dheeman, Claudio Accarino, James Grant |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Single chip Male Sarcosine Biosensing Techniques/instrumentation Point-of-Care Systems Biomedical Engineering Biophysics Biosensing Techniques Diagnostic tools Cholesterol/blood Xanthine Choline/blood Choline Xanthine/blood 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Optical sensing Lab-On-A-Chip Devices Manchester Institute of Biotechnology Electrochemistry Humans Oxides/chemistry Chromatography Oxides General Medicine Equipment Design ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology Small molecule Cholesterol blood 030104 developmental biology Cholesterol chemistry Semiconductors 030220 oncology & carcinogenesis Sarcosine/blood Biotechnology |
| Zdroj: | Patil, S B, Dheeman, D S, Al-Rawhani, M A, Velugotla, S, Nagy, B, Cheah, B C, Grant, J P, Accarino, C, Barrett, M P & Cumming, D R S 2018, ' An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites ', Biosensors & bioelectronics, vol. 122, pp. 88-94 . https://doi.org/10.1016/j.bios.2018.09.013 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.09.013 |
| Popis: | Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose. |
| Databáze: | OpenAIRE |
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