Regulation of insulin-like growth factor binding proteins in ovarian cancer cells by oestrogen
| Autor: | James G. Jackson, Daniel D. Von Hoff, Robert F. Krywicki, Shunichi Shimasaki, Timothy W. Kozelsky, Jose A. Figueroa, Douglas Yee |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
medicine.medical_specialty Time Factors medicine.medical_treatment Cell Blotting Western Ovary Biology Insulin-like growth factor-binding protein Receptor IGF Type 1 Somatomedins Internal medicine medicine Tumor Cells Cultured Humans RNA Messenger Insulin-Like Growth Factor I Receptor Ovarian Neoplasms Messenger RNA Estradiol Growth factor medicine.disease Somatomedin Insulin-Like Growth Factor Binding Proteins Tamoxifen medicine.anatomical_structure Endocrinology Oncology biology.protein Female Ovarian cancer Carrier Proteins hormones hormone substitutes and hormone antagonists |
| Zdroj: | European journal of cancer (Oxford, England : 1990). (14) |
| ISSN: | 0959-8049 |
| Popis: | Insulin-like growth factor-I (IGF-I), its receptor and its binding proteins are expressed by ovarian cancer cells. In this study, we examined oestradiol (E2) regulation of IGF-I and IGF binding proteins (IGFBP) in an oestrogen-responsive ovarian cancer cell line, PE04. In serum-free conditions, PE04 cell monolayer growth was increased 1.64-fold by 3 nmol/l E2 compared with controls, although IGF-I mRNA levels were not increased. In contrast to IGF-I mRNA, IGFBP mRNA was regulated by E2. E2 caused a marked decrease in IGFBP-3 RNA, but IGFBP-2, -4 and -6 levels were only minimally depressed. IGFBP-5 mRNA levels were increased by E2. Tamoxifen had less effect on IGFBP mRNA regulation. Ligand blotting showed that E2 reduced IGFBP levels in conditioned media. IGFBP RNA was also detected in human ovarian tissue samples. Thus, IGFBP expression can be regulated in oestrogen-responsive ovarian cancer by E2. |
| Databáze: | OpenAIRE |
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