Expression of Stress-related Genes in a Cadmium-resistant A549 Human Cell Line
| Autor: | Jean-Pierre Soleilhavoup, Christian Vincent, Françoise Croute, Jean-Claude Murat, Fumiya Obata, Bernadette Beau, Hiroyoshi Komatsu |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
A549 cell
chemistry.chemical_classification GPX1 GPX3 Gene Expression Profiling Health Toxicology and Mutagenesis Glutathione peroxidase Glutathione reductase Glutathione Adenocarcinoma Biology Toxicology GPX5 Molecular biology GPX6 Oxidative Stress chemistry.chemical_compound chemistry Tumor Cells Cultured Humans Endoplasmic Reticulum Chaperone BiP Cadmium Oligonucleotide Array Sequence Analysis |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 68:703-718 |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287390590925447 |
| Popis: | This study was designed to explain the basis for Cd-acquired tolerance of A549 cells cultured in the presence of Cd. Thirty-day exposure of cultured human pneumocytes (A549 cell line) to 10 microM Cd was previously found to induce an acquired resistance persisting over several weeks of culture. Moreover, these Cd-resistant cells (R-cells) were found to proliferate faster than controls. No difference was found between R-cells and control cells (S-cells) concerning the basal and Cd-induced level of metallothioneins expression. However, after exposure to Cd, cell glutathione levels were unchanged in R-cells while they were either increased (at 10 microM Cd) or decreased (at 25 microM Cd) in S-cells. cDNA array analysis showed that genes encoding for (GPx1) glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase were similarly expressed in R- and S-cells, whereas the gene of (GPx2) glutathione peroxidase was overexpressed in R-cells. Most genes encoding stress proteins were similarly expressed, except for HSP27 and GRP94 genes, which were respectively under- (ratio 0.5 +/- 0.1) and over- (1.8 +/- 0.5) expressed in R-cells. Acute exposure to Cd was found to trigger the upregulation of genes encoding the chaperone proteins HSP90A, HSP27, HSP40, GRP78, HSP72, and HO-1 in S-cells. In R-cells, only HO-1 and HSP72 were overexpressed but at a lower level. This suggests that the Cd-related adverse conditions, leading to protein misfolding, are lowered in R-cells. It is likely that the upregulation of GPx2 in R-cells leads to a higher antioxidant defense in these cells. |
| Databáze: | OpenAIRE |
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