Exposure to Maghemite Nanoparticles Induces Epigenetic Alterations in Human Submandibular Gland Cells
| Autor: | Marcio José Poças-Fonseca, Mariana ColaÇo Pereira Carneiro da Cunha, João Paulo Figueiró Longo, Raphael Severino Bonadio, Ricardo Bentes Azevedo |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Submandibular Gland Biomedical Engineering Bioengineering 02 engineering and technology Vacuole medicine.disease_cause Epigenesis Genetic medicine Humans Cytotoxic T cell General Materials Science Epigenetics Cytotoxicity General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Submandibular gland Cell biology Oxidative Stress medicine.anatomical_structure DNA methylation Nanoparticles Magnetic Iron Oxide Nanoparticles Reactive Oxygen Species 0210 nano-technology Reprogramming Oxidative stress |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 20:1454-1462 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2020.16956 |
| Popis: | Even though nanotechnology has revolutionized the biomedical research, a plethora of studies debate the nanoparticles safety. In order to contribute to these studies, we evaluated the cytotoxic and epigenetic effects of maghemite nanoparticles covered with citric acid on human submandibular gland cells. Objective: This work objective was to evaluate the cytotoxic effects and epigenetic alterations induced in human salivary gland cells after treatment with maghemite nanoparticles covered with citric acid. Methods: For that, human submandibular gland cells were cultured and treated with nanoparticles for 24 or 48 hours. To assess cytotoxicity we used lactate dehydrogenase, a general oxidative stress indicator assay and microscopy. Epigenetic status was detected by colorimetric assays and the results were confirmed by quantitative polymerase chain reaction. Results: No cytotoxic effects were detected on cells exposed to up 3.0 mgFemL-1 for 48 hours, although cytoplasmic vacuoles formation was detected by light microscopy analyses. An increased generation of reactive oxygen species in cells exposed to nanoparticles was evidenced and iron clusters accumulated in the cytoplasm of treated cells. Global DNA methylation and histones H3 and H4 acetylation were also altered in response to nanoparticles exposure, thus suggesting a reprogramming of the epigenome. Transcripts accumulation analyses showed that genes related to iron metabolism and oxidative stress were upregulated, while the gene related to epigenetic reprogramming presented reduced transcript accumulation after treatment. Conclusion: We concluded that maghemite nanoparticles covered with citric acid exposure provoked several biological responses without impairment of human submandibular gland cells viability. This is the first report on the epigenetic effects of maghemite nanoparticles on this cell lineage. |
| Databáze: | OpenAIRE |
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