Nesfatin-1 in the dorsal raphe nucleus influences visceral sensitivity via 5-HT neurons in male maternally separated rats
| Autor: | Xian Ge, Hui-Ai Zhang, Qian Huang, Jie Sha, Xue-Liang Li, Nan Sang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Dorsal Raphe Nucleus
Male Serotonin medicine.medical_specialty Visceral sensitivity lcsh:Medicine Withdrawal reflex Nerve Tissue Proteins Electromyography Tryptophan Hydroxylase Article 03 medical and health sciences 0302 clinical medicine Dorsal raphe nucleus Pregnancy Internal medicine Animals Nucleobindins Medicine lcsh:Science 5-HT receptor Neurons Multidisciplinary medicine.diagnostic_test business.industry Calcium-Binding Proteins lcsh:R Tryptophan hydroxylase Immunohistochemistry Rats DNA-Binding Proteins Endocrinology Female lcsh:Q 030211 gastroenterology & hepatology business Colorectal distension 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-27592-x |
| Popis: | Nesfatin-1, a satiety molecule processed from nucleobindin2 (NUCB2), is implicated in visceral hypersensitivity in rats and colocalized with 5-hydroxytryptamine (5-HT) in the dorsal raphe nucleus (DRN). Maternal separation (MS) in rats contributes to visceral hypersensitivity via elevated expression of 5-HT in the DRN. Intracerebroventricular injection of nesfatin-1 activates DRN 5-HT neurons. In this study, A model of visceral hypersensitivity was developed by subjecting rats to MS. Colorectal distension was used to detect visceral sensitivity, which was evaluated by abdominal withdrawal reflex (AWR) scores and electromyogram (EMG) magnitude. MS rats exhibited higher AWR scores and EMG magnitude compared with controls. The numbers of nesfatin-1- and tryptophan hydroxylase (TPH, the rate-limiting enzyme for 5-HT synthesis)-positive cells in the DRN were significantly elevated accordingly. Visceral hypersensitivity was significantly alleviated in MS rats treated with intra-DRN administration of anti-nesfatin-1/NUCB2, accompanied by decreased expression of 5-HT and TPH in the DRN, compared with the vehicle-treated group. In contrast, intra-DRN administration of nesfatin-1 into normal adult rats induced visceral hypersensitivity, which correlated with elevated expression of 5-HT and TPH in the DRN. In conclusion, Nesfatin-1 has critical effects on visceral hypersensitivity; the underlying mechanisms might be related to the activation of DRN 5-HT neurons. |
| Databáze: | OpenAIRE |
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