Cell-laden alginate dialdehyde–gelatin hydrogels formed in 3D printed sacrificial gel
| Autor: | Supachai Reakasame, Dalia Dranseikiene, Dirk W. Schubert, Stefan Schrüfer, Aldo R. Boccaccini |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
food.ingredient Alginates Cell Survival Tissue Engineering Constructs and Cell Substrates Cell Biomedical Engineering Biophysics Bioengineering macromolecular substances Gelatin Biomaterials food Cell Line Tumor medicine Humans Cell encapsulation Incubation Dissolution Cell Proliferation Aldehydes Tissue Engineering Tissue Scaffolds Viscosity technology industry and agriculture Bioprinting Hydrogels Dynamic mechanical analysis Casting Cross-Linking Reagents medicine.anatomical_structure Chemical engineering Barium Printing Three-Dimensional Self-healing hydrogels Calcium ddc:620 |
| Zdroj: | Journal of Materials Science. Materials in Medicine |
| Popis: | Alginate dialdehyde–gelatin (ADA–GEL) hydrogels have been reported to be suitable matrices for cell encapsulation. In general, application of ADA–GEL as bioink has been limited to planar structures due to its low viscosity. In this work, ring shaped constructs of ADA–GEL hydrogel were fabricated by casting the hydrogel into sacrificial molds which were 3D printed from 9% methylcellulose and 5% gelatin. Dissolution of the supporting structure was observed during the 1st week of sample incubation. In addition, the effect of different crosslinkers (Ba2+ and Ca2+) on the physicochemical properties of ADA–GEL and on the behavior of encapsulated MG-63 cells was investigated. It was found that Ba2+ crosslinked network had more than twice higher storage modulus, and mass decrease to 70% during incubation compared to 42% in case of hydrogels crosslinked with Ca2+. In addition, faster increase in cell viability during incubation and earlier cell network formation were observed after Ba2+ crosslinking. No negative effects on cell activity due to the use of sacrificial materials were observed. The approach presented here could be further developed for cell-laden ADA–GEL bioink printing into complex 3D structures. |
| Databáze: | OpenAIRE |
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