MicroRNA-144-3p suppresses tumor growth and angiogenesis by targeting SGK3 in hepatocellular carcinoma

Autor: Yan Feng, Rong Liang, Chaoyuan Huang, Xiaoling Luo, Xin-Ping Huang, Manya Wu
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Vascular Endothelial Growth Factor A
microRNA-144-3p
Cancer Research
Carcinoma
Hepatocellular
Angiogenesis
growth
Biology
Protein Serine-Threonine Kinases
Disease-Free Survival
Neovascularization
03 medical and health sciences
Mice
Phosphatidylinositol 3-Kinases
angiogenesis
0302 clinical medicine
Cell Movement
microRNA
medicine
Animals
Humans
PI3K/AKT/mTOR pathway
Cell Proliferation
serum and glucocorticoid kinase 3
Oncogene
Neovascularization
Pathologic
TOR Serine-Threonine Kinases
Liver Neoplasms
General Medicine
Articles
hepatocellular carcinoma
Cell cycle
medicine.disease
Molecular medicine
Xenograft Model Antitumor Assays
digestive system diseases
Gene Expression Regulation
Neoplastic
MicroRNAs
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Hepatocellular carcinoma
Cancer research
Female
prognosis
medicine.symptom
Zdroj: Oncology Reports
ISSN: 1791-2431
Popis: In our previous studies, the Illumine Soledad massively parallel signature sequencing of miRNomes in non-tumor and hepatocellular carcinoma (HCC) tissues revealed that microRNA (miR)-144-3p was significantly downregulated in HCC, but its role in HCC development, especially angiogenesis, remains unclear. In this investigation, we found recovering miR-144-3p expression can significantly suppress the growth, migration and induced angiogenic capacity of HCC cells through both in vivo and in vitro experiments. Moreover, clinical correlation analysis showed that low expression of miR-144-3p was positively correlated to poor disease-free survival (DFS) of HCC patients. Mechanistically, serum and glucocorticoid kinase 3 (SGK3), the putative targets of miR-144-3p, was predicted by Target Scan database and identified to be suppressed by miR-144-3p so that inhibiting the activation of mTOR-VEGF downstream signals was activated by the phosphoinositide 3-kinase (PI3K)-independent pathway. Hence, we concluded that miR-144-3p, which is frequently downregulated in HCC, can inhibit proliferation, migration and repress angiogenesis by regulating SGK3 activation with PI3K independent signal pathway, and acts as a prognostic factor for HCC patients.
Databáze: OpenAIRE
Externí odkaz: